复制蛋白A的中间亚基在复制猴病毒40染色体时与正在生长的RNA-DNA引物接触。
The middle subunit of replication protein A contacts growing RNA-DNA primers in replicating simian virus 40 chromosomes.
作者信息
Mass G, Nethanel T, Kaufmann G
机构信息
Department of Biochemistry, Tel Aviv University, Ramat Aviv 69978, Israel.
出版信息
Mol Cell Biol. 1998 Nov;18(11):6399-407. doi: 10.1128/MCB.18.11.6399.
Abstract
The eukaryotic single-stranded DNA binding protein replication protein A (RPA) participates in major DNA transactions. RPA also interacts through its middle subunit (Rpa2) with regulators of the cell division cycle and of the response to DNA damage. A specific contact between Rpa2 and nascent simian virus 40 DNA was revealed by in situ UV cross-linking. The dynamic attributes of the cross-linked DNA, its size distribution, its RNA primer content, and its replication fork polarity were determined [corrected]. These data suggest that Rpa2 contacts the early DNA chain intermediates synthesized by DNA polymerase alpha-primase (RNA-DNA primers) but not more advanced products. Possible signaling functions of Rpa2 are discussed, and current models of eukaryotic lagging-strand DNA synthesis are evaluated in view of our results.
摘要
真核生物单链DNA结合蛋白复制蛋白A（RPA）参与主要的DNA事务。RPA还通过其中亚基（Rpa2）与细胞分裂周期和DNA损伤应答的调节因子相互作用。通过原位紫外线交联揭示了Rpa2与新生猿猴病毒40 DNA之间的特定接触。确定了交联DNA的动态属性、其大小分布、其RNA引物含量及其复制叉极性[已校正]。这些数据表明，Rpa2与由DNA聚合酶α-引发酶合成的早期DNA链中间体（RNA-DNA引物）接触，但不与更高级的产物接触。讨论了Rpa2可能的信号传导功能，并根据我们的结果评估了当前真核生物滞后链DNA合成模型。
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