Latha B, Ramakrishnan M, Jayaraman V, Babu M
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, India.
Burns. 1998 Sep;24(6):532-8. doi: 10.1016/s0305-4179(98)00066-7.
This study was mainly aimed to investigate the efficacy of trypsin:chymotrypsin to elicit anti-oxidant properties. In our earlier studies it was observed that the enzyme preparation exhibited an anti-inflammatory action as there was a remarkable reduction in oedema formation and tissue destruction. This led to further study on the amount of lipid peroxidation products formed and the levels of enzymatic and non-enzymatic anti-oxidants and relative trace element contents of copper, selenium, iron and zinc during administration of the enzyme preparation. Decreased formation of lipid peroxidation products was observed in treated group in comparison with the untreated group. Higher levels of enzymatic anti-oxidants mainly super oxide dismutase, catalase, glutathione peroxidase and glutathione-s-transferase and non-enzymatic antioxidant namely ceruloplasmin persisted for a longer period of time in the treated group than in the untreated group. No statistical significance was observed in non-enzymatic antioxidants viz. ascorbic acid and tocopherol levels in both the groups. Increased serum copper and selenium levels in the treated group could be related to higher levels of the ceruloplasmin and glutathione peroxidase observed in the treated group. The above studies support the finding that treatment with the enzyme preparation reduced tissue destruction leading to decreased formation of free radicals and subsequent effective scavenging of free radicals by the higher levels of enzymatic and non-enzymatic anti-oxidants.
本研究主要旨在探究胰蛋白酶与胰凝乳蛋白酶引发抗氧化特性的功效。在我们早期的研究中观察到，该酶制剂表现出抗炎作用，因为水肿形成和组织破坏显著减少。这促使我们进一步研究在给予酶制剂期间脂质过氧化产物的生成量、酶促和非酶促抗氧化剂的水平以及铜、硒、铁和锌等相关微量元素的含量。与未处理组相比，处理组中脂质过氧化产物的生成减少。与未处理组相比，处理组中酶促抗氧化剂（主要是超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽 - S - 转移酶）和非酶促抗氧化剂（即铜蓝蛋白）的水平在较长时间内持续较高。两组中非酶促抗氧化剂（即抗坏血酸和生育酚）的水平未观察到统计学差异。处理组血清铜和硒水平的升高可能与处理组中观察到的较高水平的铜蓝蛋白和谷胱甘肽过氧化物酶有关。上述研究支持了以下发现：用酶制剂进行治疗可减少组织破坏，从而导致自由基生成减少，随后较高水平的酶促和非酶促抗氧化剂可有效清除自由基。
