Sepúlveda-Saavedra J, Bermúdez de Rocha M V, Tamez-Rodríguez V A, Ballesteros-Elizondo R G, Moreno-Sepúlveda M, Piñeyro-López A
Departamento de Histologia, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico.
Toxicol Lett. 1998 Sep 1;98(1-2):71-5. doi: 10.1016/s0378-4274(98)00104-0.
Peroxisomes are single-membrane-bound organelles present in almost all eukaryotic cells. Hypolipidemic agents such as clofibrate, herbicides and plasticizers induce an increase in the number and size of peroxisomes from mammalian cells. However, there is no evidence of drugs causing a decrease in the number of these organelles. In this paper, we report the effect in vivo of toxin T-514 extracted from the plant Karwinskia humboldtiana, now re-named peroxisomicine-A1, on hepatic peroxisomes from rats intoxicated with this compound. Rats were treated with a single dose of 25 mg/kg of peroxisomicine-A1 and at different times were killed by decapitation. For the peroxisomal counting, liver tissue sections from control and treated rats were processed for the localization of catalase in peroxisomes. The results of the quantitative analysis demonstrated a significant decrease in the number of liver peroxisomes from rats intoxicated with peroxisomicine-A1. This finding suggests that peroxisomicine-A1 as in yeast, causes a damage to mammalian peroxisomes. The diminution in the number of peroxisomes could be a consequence of damage to the organelle, which is further removed by an autophagic process.
过氧化物酶体是几乎存在于所有真核细胞中的单膜结合细胞器。降脂药物如氯贝丁酯、除草剂和增塑剂会使哺乳动物细胞中的过氧化物酶体数量增加、体积增大。然而，尚无证据表明药物会导致这些细胞器数量减少。在本文中，我们报告了从现已重新命名为过氧化物酶体素 -A1的植物洪堡卡氏藤中提取的毒素T-514对用该化合物中毒的大鼠肝脏过氧化物酶体的体内作用。给大鼠单次注射25 mg/kg的过氧化物酶体素 -A1，并在不同时间断头处死。为了进行过氧化物酶体计数，对对照大鼠和处理大鼠的肝脏组织切片进行处理，以定位过氧化物酶体中的过氧化氢酶。定量分析结果表明，用过氧化物酶体素 -A1中毒的大鼠肝脏过氧化物酶体数量显著减少。这一发现表明，过氧化物酶体素 -A1与在酵母中一样，会对哺乳动物的过氧化物酶体造成损害。过氧化物酶体数量的减少可能是细胞器受损的结果，受损细胞器会通过自噬过程被进一步清除。
