Giesen U, Kleider W, Berding C, Geiger A, Orum H, Nielsen P E
Boehringer Mannheim GmbH, Roche Diagnostics, Business Unit Laboratory Systems, Bahnhofstr. 9-15,82327 Tutzing, Germany.
Nucleic Acids Res. 1998 Nov 1;26(21):5004-6. doi: 10.1093/nar/26.21.5004.
An empirical formula for thermal stability (T m) prediction of PNA/DNA duplexes has been derived. The model is based on the T m as calculated for the corresponding DNA/DNA duplex employing a nearest neighbour approach, by including terms for the pyrimidine content and length of the PNA to take into account the increased thermostability of PNA/DNA hybrids and the asymmetry of the PNA-DNA heteroduplex. The predictive power of the T m prediction formula was challenged with an independent data set not used for model building. The T m of >90% of the sequences was predicted within 5 K; 98% of the predicted T ms differ by not more than 10 K from the experimentally determined T m.
已推导了一种用于预测肽核酸(PNA)/DNA双链体热稳定性(熔解温度Tm)的经验公式。该模型基于使用最近邻方法计算相应DNA/DNA双链体的Tm,并纳入了嘧啶含量和PNA长度的项,以考虑PNA/DNA杂交体增加的热稳定性以及PNA-DNA异源双链体的不对称性。通过一个未用于模型构建的独立数据集对Tm预测公式的预测能力进行了验证。超过90%的序列的Tm预测值在5 K范围内;98%的预测Tm与实验测定的Tm相差不超过10 K。