Sugimoto N, Yamamoto K, Satoh N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symp Ser. 1999(42):95-6. doi: 10.1093/nass/42.1.95.
We report positional effect of bulge nucleotide on PNA/DNA hybrid stability. CD spectra showed that PNA/DNA hybrids required at least seven base pairings at a stem region to form a bulged structure. On the other hand, DNA/DNA could form bulged structure when there are only four base pairings adjacent to the bulge nucleotide. We discuss why PNA requests such a many base pairings to form bulged structure from a nearest neighbor standpoint.
我们报告了凸起核苷酸对肽核酸（PNA）/DNA杂交稳定性的位置效应。圆二色光谱表明，PNA/DNA杂交体在茎区至少需要七个碱基对来形成凸起结构。另一方面，当与凸起核苷酸相邻仅有四个碱基对时，DNA/DNA就能形成凸起结构。我们从最近邻角度讨论了为什么PNA形成凸起结构需要这么多碱基对。
