Shenker B, Gray I
J Immunol Methods. 1976;13(2):161-6. doi: 10.1016/0022-1759(76)90154-x.
A dextran density gradient centrifugation method has been used to separate PHA transformed lymphocytes from nontransformed lymphocytes obtained from rat blood. Fifty-four percent of the transformed lymphocytes were recovered in the fraction at a density of 1.0620 g/ml. Eighty-five percent of the cells in this fraction were transformed cells while the remainder were non-transformed lymphocytes.
已采用葡聚糖密度梯度离心法从大鼠血液中获取的未转化淋巴细胞中分离出PHA转化淋巴细胞。54%的转化淋巴细胞在密度为1.0620 g/ml的组分中回收。该组分中85%的细胞为转化细胞，其余为未转化淋巴细胞。
