Biermann A, von Keyserlingk D G, Boll I
Blut. 1977 Jul 20;35(1):57-64. doi: 10.1007/BF01006965.
Venous blood from eight healthy subjects was divided into four fractions on a discontinuous albumin density gradient. The percentage recovery of lymphocytes was 82.3%; the purity of the lymphocyte fractions was 83.6%. The lymphocytes were cultured with PHA and Endotoxin, and the samples were analysed after 24, 48, and 72 hours. After PHA stimulation immunoblasts appeared up to 59.3% in the cultures from the 19-21% albumin fraction. After Endotoxin stimulation the maximum (75.8%) was reached in the heavy (25-27% albumin) fraction. Thus, it is concluded that the lymphocytes which can be stimulated with both the mitogens have different densities, the PHA-stimulable T lymphocytes being ligther than the Endotoxin-stimulable B lymphocytes. It is also concluded that as a mitogen Endotoxin is equal to PHA.
从八名健康受试者采集的静脉血在不连续白蛋白密度梯度上被分成四个部分。淋巴细胞的回收率为82.3%；淋巴细胞部分的纯度为83.6%。淋巴细胞用PHA和内毒素培养，在24、48和72小时后对样本进行分析。PHA刺激后，来自19 - 21%白蛋白部分的培养物中免疫母细胞出现率高达59.3%。内毒素刺激后，在重（25 - 27%白蛋白）部分达到最大值（75.8%）。因此，得出结论：能被两种有丝分裂原刺激的淋巴细胞具有不同密度，PHA可刺激的T淋巴细胞比内毒素可刺激的B淋巴细胞轻。还得出结论：作为一种有丝分裂原，内毒素与PHA相当。
