Quayle A J, Coston W M, Trocha A K, Kalams S A, Mayer K H, Anderson D J
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
J Immunol. 1998 Oct 15;161(8):4406-10.
CTLs play an important role in controlling cell-associated HIV. Since the majority of HIV infections are acquired through sexual transmission, we investigated whether antiviral CTLs were present in the male urogenital tract using semen as a source of T cells. We were able to establish anti-HIV cytolytic lines in five of five HIV-infected men with CD4 counts of >500/microl, although cloning efficiencies were lower than with peripheral blood-derived T cells. CTLs generated from the semen of three men were analyzed in detail and showed a broadly active response, recognizing gag, env, and pol proteins. Detailed analysis of two gag-specific clones from one of the individuals demonstrated HLA class I restriction and recognition of the same p24 epitope (EQASQEVKNWMT). In summary, our results demonstrate the presence of a broad CTL response to HIV in the urogenital tract and provide a rationale for further studies of local enhancement of genital mucosal responses by anti-HIV immunization.
细胞毒性T淋巴细胞（CTLs）在控制与细胞相关的HIV方面发挥着重要作用。由于大多数HIV感染是通过性传播获得的，我们以精液作为T细胞来源，研究了男性泌尿生殖道中是否存在抗病毒CTLs。我们能够在五名CD4计数>500/微升的HIV感染男性中的五人中建立抗HIV细胞溶解系，尽管克隆效率低于外周血来源的T细胞。对三名男性精液产生的CTLs进行了详细分析，结果显示出广泛的活性反应，可识别gag、env和pol蛋白。对其中一名个体的两个gag特异性克隆进行的详细分析表明，其具有HLA I类限制性，并识别相同的p24表位（EQASQEVKNWMT）。总之，我们的结果证明了泌尿生殖道中存在对HIV的广泛CTL反应，并为通过抗HIV免疫进一步研究局部增强生殖器黏膜反应提供了理论依据。
