Uzumcu M, Braileanu G T, Carnahan K G, Ludwig T E, Mirando M A
Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-6353, USA.
Biol Reprod. 1998 Nov;59(5):1259-65. doi: 10.1095/biolreprod59.5.1259.
Oxytocin (OT) is the physiological stimulus for pulsatile release of endometrial prostaglandin (PG) F2alpha during luteolysis in domestic ungulates, and the cellular mechanism for this appears to involve phosphoinositide (PI) hydrolysis. To determine which endometrial cell type(s) was responsive to OT during luteolysis in swine, luminal epithelial (LEC), glandular epithelial (GEC), and stromal cells (SC) were isolated from endometrium by differential enzymatic digestion and sieve filtration on Day 16 postestrus and cultured. For PI hydrolysis in experiment 1, SC were most responsive to 100 nM OT (p < 0.001), whereas LEC were least responsive and GEC had an intermediate response (p < 0.001). For PGF secretion in experiment 2, the response to OT was greatest for SC, least for LEC, and intermediate for GEC. In experiment 3, 100 nM OT increased PI hydrolysis in SC within 30 min (p < 0.05) and in GEC within 60 min (p < 0.05) but did not increase PI hydrolysis in LEC. In experiment 4, PI hydrolysis in SC was increased (p < 0.05) by 33-333 nM OT but was not increased by </= 333 nM OT in GEC or LEC after 30 min. However, PGF secretion from SC was increased (p < 0.05) by 10-333 nM OT, and from GEC by 10-333 nM OT, but was not increased from LEC by </= 333 nM OT. Results of this study indicate that 1) there was differential responsiveness to OT among endometrial cell types, and 2) within cell type, there generally was a similar response to OT for both PI hydrolysis and PGF secretion, further implicating PI hydrolysis as the signaling pathway for OT-stimulated PGF2alpha release. The differential response of endometrial cell types may have an important role in the pattern of PGF2alpha secretion during luteolysis in swine.
在家畜黄体溶解过程中，催产素（OT）是子宫内膜前列腺素（PG）F2α脉冲式释放的生理刺激因素，而这一过程的细胞机制似乎涉及磷酸肌醇（PI）水解。为了确定在猪黄体溶解过程中哪种子宫内膜细胞类型对OT有反应，在发情期后第16天，通过差异酶消化和筛网过滤从子宫内膜中分离出腔上皮细胞（LEC）、腺上皮细胞（GEC）和基质细胞（SC）并进行培养。在实验1中，对于PI水解，SC对100 nM OT反应最为强烈（p < 0.001），而LEC反应最弱，GEC反应介于两者之间（p < 0.001）。在实验2中，对于PGF分泌，SC对OT的反应最大，LEC最小，GEC介于两者之间。在实验3中，100 nM OT在30分钟内增加了SC中的PI水解（p < 0.05），在60分钟内增加了GEC中的PI水解（p < 0.05），但未增加LEC中的PI水解。在实验4中，33 - 333 nM OT在30分钟后增加了SC中的PI水解（p < 0.05），但在GEC或LEC中，≤333 nM OT并未增加PI水解。然而，10 - 333 nM OT增加了SC中的PGF分泌（p < 0.05），10 - 333 nM OT增加了GEC中的PGF分泌，但≤333 nM OT未增加LEC中的PGF分泌。本研究结果表明：1）子宫内膜细胞类型对OT的反应存在差异；2）在细胞类型内，PI水解和PGF分泌对OT的反应通常相似，这进一步表明PI水解是OT刺激PGF2α释放的信号通路。子宫内膜细胞类型的差异反应可能在猪黄体溶解过程中PGF2α分泌模式中起重要作用。
