Verastegui M, Moro P, Guevara A, Rodriguez T, Miranda E, Gilman R H
Department of Microbiology, Cayetano Heredia University, Lima, Peru.
J Clin Microbiol. 1992 Jun;30(6):1557-61. doi: 10.1128/jcm.30.6.1557-1561.1992.
Sera from 71 patients with surgically confirmed hydatid disease (which is caused by Echinococcus granulosus) were studied by an enzyme-linked immunoelectrotransfer blot (EITB) assay. Sera from patients either with other cestode infections or with another illness were used as controls. Results of the EITB test for hydatidosis were compared with those of the double-diffusion (DD5) test and an enzyme-linked immunosorbent assay (ELISA). In the EITB assay with bovine lyophilized hydatid fluid, three antigen bands of 8, 16, and 21 kDa were diagnostically important. The sensitivity of the assay by using these antigen bands was 80% for hepatic cysts, 56% for pulmonary cysts, and 56% for cysts located in multiple organs. In sera from controls, the specificity of the EITB assay was 100%. Cross-reactions to the 8-, 16-, and 21-kDa bands occurred, respectively, in 12, 4, and 4% of sera from patients with cysticercosis. No cross-reactions were noted in patients infected with Hymenolepis nana. The ELISA in which swine hydatid fluid was used as the antigen was as sensitive as the EITB test but was less specific (80%) and frequently cross-reacted with sera from patients with other cestode infections. The sensitivity of the DD5 test, which uses sheep hydatid fluid, was low (47%) , but its specificity was as high as that of the EITB assay. However, in patients with cysticercosis, cross-reactions were observed in 23% of sera tested. Despite the higher sensitivity found with the EITB assay, 23% (n = 5) of the serum samples that were positive by the DD5 test were not detected by the EITB assay. The EITB assay offers greater sensitivity and specificity than do the ELISA and the DD5 test. The highest proportion of hydatid cases is detected when the EITB and DD5 tests are run simultaneously.
采用酶联免疫电转移印迹法（EITB）对71例经手术确诊为包虫病（由细粒棘球绦虫引起）患者的血清进行了研究。将患有其他绦虫感染或其他疾病患者的血清用作对照。将包虫病的EITB检测结果与双向扩散试验（DD5）和酶联免疫吸附试验（ELISA）的结果进行了比较。在用牛冻干包虫液进行的EITB检测中，8、16和21 kDa的三条抗原带具有诊断意义。使用这些抗原带进行检测时，该检测方法对肝囊肿的敏感性为80%，对肺囊肿为56%，对位于多个器官的囊肿为56%。在对照血清中，EITB检测的特异性为100%。囊尾蚴病患者血清中分别有12%、4%和4%与8、16和21 kDa条带发生交叉反应。在感染微小膜壳绦虫的患者中未发现交叉反应。以猪包虫液为抗原的ELISA与EITB检测一样敏感，但特异性较低（80%），且经常与其他绦虫感染患者的血清发生交叉反应。使用绵羊包虫液的DD5检测的敏感性较低（47%），但其特异性与EITB检测一样高。然而，在囊尾蚴病患者中，23%的检测血清出现了交叉反应。尽管EITB检测具有更高的敏感性，但DD5检测呈阳性的血清样本中有23%（n = 5）未被EITB检测发现。与ELISA和DD5检测相比，EITB检测具有更高的敏感性和特异性。同时进行EITB和DD5检测时，检测到的包虫病病例比例最高。
