Ondrey F G, Juhn S K, Adams G L
Tumor Cell Biology Division/Head and Neck Surgery Branch, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, USA.
Otolaryngol Head Neck Surg. 1998 Oct;119(4):342-5. doi: 10.1016/S0194-5998(98)70075-0.
Various cytokines are presently known to be associated with the regulation of inflammatory responses. In pediatric otitis media, cytokines that correlate with various degrees of inflammation are present in middle ear effusions as inflammatory mediators. The present study was undertaken to examine the potential role of the early-response cytokines, interleukin-1beta and tumor necrosis factor-alpha, in adult otitis media. Fifty-nine adults with otitis media underwent tympanocentesis, and the effusion specimens were analyzed for the presence of both cytokines by enzyme-linked immunosorbent assay methods. Eighty-eight percent of the effusions were serous in nature. Sixty-seven percent of the patients had a known history of head and neck malignancy and radiation to the temporal bone. Twelve percent of the effusions were positive for interleukin-1beta expression, compared with 85% of effusions in children with otitis media. Eight percent of the effusions contained tumor necrosis factor-alpha, compared with 85% of those collected in pediatric otitis media. All of the specimens that contained tumor necrosis factor-alpha also contained interleukin-1beta. In the present study, there was no correlation with head and neck malignancy/radiation or the clinical degree of inflammation with the presence of either cytokine. We conclude that adult otitis media is associated with lower expression of an acute inflammatory response, as judged by the levels of interleukin-1beta and tumor necrosis factor-alpha in the effusions. Additionally, adult otitis probably represents a less severe and more chronic inflammatory state in comparison with pediatric otitis media. Further analysis of inflammatory mediators in adult otitis media is necessary to evaluate the contribution of cytokines in relation to various etiologic factors.
目前已知多种细胞因子与炎症反应的调节有关。在小儿中耳炎中，与不同程度炎症相关的细胞因子作为炎症介质存在于中耳积液中。本研究旨在探讨早期反应细胞因子白细胞介素-1β和肿瘤坏死因子-α在成人中耳炎中的潜在作用。59例成人中耳炎患者接受了鼓膜穿刺术，并通过酶联免疫吸附测定法分析积液标本中这两种细胞因子的存在情况。88%的积液本质上是浆液性的。67%的患者有头颈部恶性肿瘤病史且颞骨接受过放疗。12%的积液白细胞介素-1β表达呈阳性，而小儿中耳炎患者积液的这一比例为85%。8%的积液含有肿瘤坏死因子-α，小儿中耳炎患者积液的这一比例为85%。所有含有肿瘤坏死因子-α的标本也都含有白细胞介素-1β。在本研究中，无论是细胞因子的存在与否，均与头颈部恶性肿瘤/放疗或炎症的临床程度无关。我们得出结论，根据积液中白细胞介素-1β和肿瘤坏死因子-α的水平判断，成人中耳炎与急性炎症反应的较低表达有关。此外，与小儿中耳炎相比，成人中耳炎可能代表一种不太严重且更慢性的炎症状态。有必要对成人中耳炎中的炎症介质进行进一步分析，以评估细胞因子与各种病因因素的关系。
