Ito S, Kobayashi T, Ara K, Ozaki K, Kawai S, Hatada Y
Biological Science Laboratory of Kao Corporation, Tochigi, Japan.
Extremophiles. 1998 Aug;2(3):185-90. doi: 10.1007/s007920050059.
The cleaning power of detergents seems to have peaked; all detergents contain similar ingredients and are based on similar detergency mechanisms. To improve detergency, modern types of heavy-duty powder detergents and automatic dishwasher detergents usually contain one or more enzymes, such as protease, amylase, cellulase, and lipase. Alkaliphilic Bacillus strains are often good sources of alkaline extracellular enzymes, the properties of which fulfil the essential requirements for enzymes to be used in detergents. We have isolated numbers of alkaliphilic Bacillus that produce such alkaline detergent enzymes, including cellulase (CMCase), protease, alpha-amylase, and debranching enzymes, and have succeeded in large-scale industrial production of some of these enzymes. Here, we describe the enzymatic properties, genetics, and structures of the detergent enzymes that we have developed.
洗涤剂的清洁能力似乎已达到顶峰；所有洗涤剂都含有相似的成分，并基于相似的去污机制。为了提高去污能力，现代类型的重型粉末洗涤剂和自动洗碗机洗涤剂通常含有一种或多种酶，如蛋白酶、淀粉酶、纤维素酶和脂肪酶。嗜碱芽孢杆菌菌株通常是碱性胞外酶的良好来源，其特性满足了用于洗涤剂的酶的基本要求。我们已经分离出许多产生此类碱性洗涤剂酶的嗜碱芽孢杆菌，包括纤维素酶（羧甲基纤维素酶）、蛋白酶、α-淀粉酶和脱支酶，并成功地对其中一些酶进行了大规模工业生产。在此，我们描述我们所开发的洗涤剂酶的酶学性质、遗传学和结构。
