Vivien-Roels B, Malan A, Rettori M C, Delagrange P, Jeanniot J P, Pévet P
UMR CNRS 7518, Université Louis Pasteur, Strasbourg, France.
J Biol Rhythms. 1998 Oct;13(5):403-9. doi: 10.1177/074873098129000228.
Melatonin was measured using a specific radioimmunoassay in 1 strain of outbred mice (OF1 Swiss) and 4 strains of inbred mice, 2 of them being known to synthesize melatonin (CBA and C3H) and the 2 others being controversial (BALB/c and C57BL/6). In this study, the 5 mouse strains were able to synthesize melatonin, but the basal levels as well as the diurnal variations were very different from one strain to another. CBA and C3H strains showed a clear-cut day-night rhythm of pineal melatonin concentration, with peak levels of 276 +/- 22 pg/pineal in CBA and 135 +/- 12 pg/pineal in C3H. In BALB/c, the authors confirmed the presence of a very short melatonin peak (15 min) in the middle of the dark period. In C57BL/6 and OF1 Swiss, a very small but significant peak was observed in the middle of the darkness. In the former, another small peak was also observed at light onset. Whether these very small peaks, which may be related to the deficience of N-acetyl transferase activity reported by others, have a physiological meaning remains to be determined.
采用特异性放射免疫分析法对1个远交系小鼠品系（OF1瑞士小鼠）和4个近交系小鼠品系进行褪黑素测定，其中已知2个品系（CBA和C3H）能合成褪黑素，另外2个品系（BALB/c和C57BL/6）存在争议。在本研究中，这5个小鼠品系均能合成褪黑素，但不同品系之间的基础水平以及昼夜变化差异很大。CBA和C3H品系显示出松果体褪黑素浓度明显的昼夜节律，CBA品系的峰值水平为276±22 pg/松果体，C3H品系为135±12 pg/松果体。在BALB/c品系中，作者证实了在黑暗期中间存在一个非常短暂的褪黑素峰值（15分钟）。在C57BL/6和OF1瑞士小鼠中，在黑暗期中间观察到一个非常小但显著的峰值。在前者中，在光照开始时也观察到另一个小峰值。这些可能与其他人报道的N-乙酰转移酶活性缺乏有关的非常小的峰值是否具有生理意义仍有待确定。
