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[培养基pH值对肠杆菌群体诱导自溶的影响]

[The effect of the pH of the medium on induced autolysis in populations of enterobacteria].

作者信息

Akaĭzin E S

机构信息

State Medical Academy, Ivanovo, Russia.

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1998 Jul-Aug(4):6-8.

PMID:9783388
Abstract

Changes in pH in the process of the induced autolysis of salmonellae and Escherichia coli were studied. The induced autolysis of enterobacterial populations was studied in connection with the acidity values of the medium in which the process was carried out. The pH of extracellular fluid, the optical density of cell suspension, the content of total protein and amino nitrogen were analyzed. Enterobacterial populations were found to be capable of the self-regulation of the acidity of the medium in the process of induced autolysis. The study revealed that the most Intensive autolysis of bacteria took place when the process was carried out in extreme alkaline values of pH.

摘要

研究了沙门氏菌和大肠杆菌诱导自溶过程中的pH变化。结合进行该过程的培养基的酸度值,研究了肠杆菌群体的诱导自溶。分析了细胞外液的pH、细胞悬液的光密度、总蛋白和氨基氮的含量。发现肠杆菌群体在诱导自溶过程中能够对培养基的酸度进行自我调节。研究表明,当在极端碱性的pH值下进行该过程时,细菌的自溶最为强烈。

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