Banker R, Carmeli S
School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.
J Nat Prod. 1998 Oct;61(10):1248-51. doi: 10.1021/np980138j.
Four modified cyclic hexapeptides, tenuecyclamides A-D (1-4), were isolated along with the known antibiotic, borophycin (5), from the methanol extract of Nostoc spongiaeforme var. tenue (TAU strain IL-184-6). The planar structure of tenuecyclamides A-D (1-4) was determined by homonuclear and inverse-heteronuclear 2D-NMR techniques as well as by high-resolution mass spectrometry measurements. The absolute configuration of the asymmetric centers was studied by Marfey's method for HPLC. The stereochemistry of the asymmetric centers in tenuecyclamides A and B (1 and 2) could not be fully determined, while that of tenuecyclamides C and D (3 and 4) was unambiguously determined.
从细颤鱼腥藻变种（TAU菌株IL-184-6）的甲醇提取物中，分离得到了四种修饰的环状六肽，即细环酰胺A-D（1-4），以及已知抗生素硼霉素（5）。通过同核和异核二维核磁共振技术以及高分辨率质谱测量确定了细环酰胺A-D（1-4）的平面结构。采用马尔菲法（Marfey's method）进行高效液相色谱分析，研究了不对称中心的绝对构型。细环酰胺A和B（1和2）中不对称中心的立体化学无法完全确定，而细环酰胺C和D（3和4）的立体化学则已明确确定。
