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布鲁氏菌流产亚种血清学检测呈阳性的牛，体内含有抗布鲁氏菌流产亚种铜锌超氧化物歧化酶的抗体。

Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase.

作者信息

Tabatabai L B, Hennager S G

机构信息

National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA.

出版信息

Clin Diagn Lab Immunol. 1994 Sep;1(5):506-10. doi: 10.1128/cdli.1.5.506-510.1994.

DOI:10.1128/cdli.1.5.506-510.1994
PMID:8556493
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368323/
Abstract

In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing native Cu-Zn superoxide dismutase and with recombinant B. abortus Cu-Zn superoxide dismutase. The results represent a first step in the direction of the development of a multiprotein diagnostic reagent for bovine brucellosis.

摘要

在本研究中，我们通过铜锌超氧化物歧化酶特异性酶联免疫测定法证明，布鲁氏菌流产亚种血清学阳性的牛对布鲁氏菌流产亚种铜锌超氧化物歧化酶具有血清免疫球蛋白G抗体。通过用含有天然铜锌超氧化物歧化酶的布鲁氏菌流产亚种盐提取物蛋白和重组布鲁氏菌流产亚种铜锌超氧化物歧化酶进行蛋白质印迹（免疫印迹）分析，证实了抗体反应的特异性。这些结果代表了牛布鲁氏菌病多蛋白诊断试剂开发方向上的第一步。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cf/368323/ddee9af82bb9/cdli00005-0035-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cf/368323/34e909c24179/cdli00005-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cf/368323/ddee9af82bb9/cdli00005-0035-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cf/368323/34e909c24179/cdli00005-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cf/368323/ddee9af82bb9/cdli00005-0035-b.jpg

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本文引用的文献

1
Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3.流产布鲁氏菌1119 - 3的抗原性S型脂多糖
Infect Immun. 1984 Nov;46(2):384-8. doi: 10.1128/iai.46.2.384-388.1984.
2
Specific enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus.用于检测牛抗流产布鲁氏菌抗体的特异性酶联免疫吸附测定
J Clin Microbiol. 1984 Aug;20(2):209-13. doi: 10.1128/jcm.20.2.209-213.1984.
3
Purification and properties of a unique superoxide dismutase from Nocardia asteroides.来自星形诺卡氏菌的一种独特超氧化物歧化酶的纯化及性质
猪布鲁氏菌脲酶的酶学、免疫学及系统发育特征
BMC Microbiol. 2008 Jul 19;8:121. doi: 10.1186/1471-2180-8-121.
4
O-Polysaccharide epitopic heterogeneity at the surface of Brucella spp. studied by enzyme-linked immunosorbent assay and flow cytometry.通过酶联免疫吸附测定和流式细胞术研究布鲁氏菌属表面的O-多糖表位异质性
Clin Diagn Lab Immunol. 1998 Nov;5(6):862-70. doi: 10.1128/CDLI.5.6.862-870.1998.
5
Effect of P39 gene deletion in live Brucella vaccine strains on residual virulence and protective activity in mice.减毒活布鲁氏菌疫苗株中P39基因缺失对小鼠残余毒力和保护活性的影响。
Infect Immun. 1998 Nov;66(11):5561-4. doi: 10.1128/IAI.66.11.5561-5564.1998.
6
Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay.在间接酶联免疫吸附测定中，布鲁氏菌感染的牛、绵羊和山羊对八种纯化的重组布鲁氏菌蛋白的体液免疫反应。
Clin Diagn Lab Immunol. 1997 Sep;4(5):556-64. doi: 10.1128/cdli.4.5.556-564.1997.
7
Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp.光滑型和粗糙型布鲁氏菌属感染中针对嗜水气单胞菌胞质和外膜抗原的抗体及迟发型超敏反应
Clin Diagn Lab Immunol. 1997 May;4(3):279-84. doi: 10.1128/cdli.4.3.279-284.1997.
8
Characterization of a monoclonal antibody specific for Brucella smooth lipopolysaccharide and development of a competitive enzyme-linked immunosorbent assay to improve the serological diagnosis of brucellosis.一种针对布鲁氏菌光滑型脂多糖的单克隆抗体的特性鉴定以及一种竞争性酶联免疫吸附测定法的开发，以改进布鲁氏菌病的血清学诊断。
Clin Diagn Lab Immunol. 1996 May;3(3):309-14. doi: 10.1128/cdli.3.3.309-314.1996.
9
Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep.用部分纯化的28千道尔顿胞质可溶性蛋白进行酶联免疫吸附测定，以对感染布鲁氏菌的绵羊和接种布鲁氏菌Rev.1疫苗的绵羊进行血清学鉴别。
Clin Diagn Lab Immunol. 1996 May;3(3):305-8. doi: 10.1128/cdli.3.3.305-308.1996.
J Biol Chem. 1983 Jan 10;258(1):91-6.
4
Role of superoxide dismutase and catalase as determinants of pathogenicity of Nocardia asteroides: importance in resistance to microbicidal activities of human polymorphonuclear neutrophils.超氧化物歧化酶和过氧化氢酶作为星状诺卡菌致病性决定因素的作用：在抵抗人多形核中性粒细胞杀菌活性中的重要性。
Infect Immun. 1985 Jan;47(1):135-41. doi: 10.1128/iai.47.1.135-141.1985.
5
The lipopolysaccharides of Brucella abortus and B. melitensis.流产布鲁氏菌和羊布鲁氏菌的脂多糖。
Ann Inst Pasteur Microbiol. 1987 Jan-Feb;138(1):92-8. doi: 10.1016/0769-2609(87)90083-4.
6
Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19.用于区分自然感染牛流产布鲁氏菌或接种19号菌株疫苗的牛抗体反应的酶联免疫吸附测定
Am J Vet Res. 1989 Jan;50(1):5-9.
7
Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase.布鲁氏菌铜锌超氧化物歧化酶的克隆、表达及存在情况
Infect Immun. 1990 Sep;58(9):2935-9. doi: 10.1128/iai.58.9.2935-2939.1990.
8
A protein isolated from Brucella abortus is a Cu-Zn superoxide dismutase.从流产布鲁氏菌中分离出的一种蛋白质是铜锌超氧化物歧化酶。
Biochemistry. 1990 Jan 16;29(2):372-6. doi: 10.1021/bi00454a010.
9
Differentiation by western blotting of immune responses of cattle vaccinated with Brucella abortus strain 19 or infected experimentally or naturally with virulent Brucella abortus.通过蛋白质印迹法对用布鲁氏菌19号菌株接种疫苗或经实验性或自然感染强毒布鲁氏菌流产亚种的牛的免疫反应进行鉴别。
Vet Microbiol. 1991 Mar;27(1):79-90. doi: 10.1016/0378-1135(91)90064-m.
10
Superoxide dismutases of virulent and avirulent strains of Brucella abortus.
Vet Microbiol. 1991 Feb 15;26(4):359-66. doi: 10.1016/0378-1135(91)90029-f.