莱姆病疏螺旋体复合群菌株的生长温度范围。
Growth temperature ranges of Borrelia burgdorferi sensu lato strains.
作者信息
Hubálek Z, Halouzka J, Heroldová M
机构信息
Institute of Landscape Ecology, Academy of Sciences, Brno, Czech Republic.
出版信息
J Med Microbiol. 1998 Oct;47(10):929-32. doi: 10.1099/00222615-47-10-929.
Three strains of Borrelia burgdorferi sensu lato, representing three human pathogenic genomospecies (B31, B. burgdorferi sensu stricto; BR14, B. garinii; BR75, B. afzelii) were grown in BSK-H medium at different temperatures and the spirochaetal cells were counted by dark-field microscopy after 0, 4, 8, 16 and 48 days. Approximate optimum (minimum-maximum) temperatures for the in-vitro growth were found to be 33 degrees C (22-39 degrees C) in strain B31, 35 degrees C (20-40 degrees C) in strain BR75 and 37 degrees C (20-41 degrees C) in strain BR14. Maximum, optimum and minimum growth temperatures seem to be important characteristics of B. burgdorferi s.l. strains, with relevance for the symptomatology, epidemiology and epizoology of Lyme borreliosis.
三种狭义伯氏疏螺旋体菌株，分别代表三种人类致病基因种（B31，狭义伯氏疏螺旋体；BR14，伽氏疏螺旋体；BR75，阿氏疏螺旋体），在不同温度下于BSK - H培养基中培养，并在0、4、8、16和48天后通过暗视野显微镜对螺旋体细胞进行计数。发现体外生长的近似最适（最低 - 最高）温度在菌株B31中为33℃（22 - 39℃），在菌株BR75中为35℃（20 - 40℃），在菌株BR14中为37℃（20 - 41℃）。最高、最适和最低生长温度似乎是狭义伯氏疏螺旋体菌株的重要特征，与莱姆病的症状学、流行病学和动物流行病学相关。
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