Putz G, Walch M, Van Eijk M, Haagsman H P
Department of Anaesthesia and Intensive Care Medicine, The Leopold Franzens University of Innsbruck, A-6020 Innsbruck, Austria.
Biophys J. 1998 Nov;75(5):2229-39. doi: 10.1016/S0006-3495(98)77667-2.
Mechanisms underlying the surface properties of lung surfactant are extensively studied in in vitro systems such as the captive-bubble surfactometer (CBS), the pulsating-bubble surfactometer, and the Wilhelmy balance. Among these systems, the CBS is advantageous when a leakproof system and high cycling rates are required. However, widespread application of the CBS to mechanistic studies of dynamic surfactant protein-phospholipid interactions of spread film and to comparative studies between spread and adsorbed film is hampered because spreading of film is difficult. In addition, when film is formed by adsorption, the amount of material required is fairly large. We have developed an easy spreading technique that allows routine formation of film by spreading of small amounts of surfactant components at the air-water interface of an air bubble in a CBS. The technique is reliable, precise, and accurate, and the biophysical activity of film formed by spreading is similar to that of film formed by adsorption. This method will be useful for mechanistic studies of surfactant components under dynamic conditions and for comparative studies of spread films and adsorbed films.
肺表面活性剂表面特性的潜在机制在体外系统中得到了广泛研究，如俘获气泡表面张力仪（CBS）、脉动气泡表面张力仪和威尔海姆天平。在这些系统中，当需要防漏系统和高循环速率时，CBS具有优势。然而，由于薄膜铺展困难，CBS在铺展薄膜的动态表面活性剂蛋白 - 磷脂相互作用的机制研究以及铺展膜与吸附膜之间的比较研究中的广泛应用受到阻碍。此外，当通过吸附形成薄膜时，所需材料量相当大。我们开发了一种简便的铺展技术，该技术允许通过在CBS中气泡的气 - 水界面上铺展少量表面活性剂成分来常规形成薄膜。该技术可靠、精确且准确，通过铺展形成的薄膜的生物物理活性与通过吸附形成的薄膜相似。这种方法将有助于在动态条件下对表面活性剂成分进行机制研究以及对铺展膜和吸附膜进行比较研究。
