Putz G, Goerke J, Taeusch H W, Clements J A
Cardiovascular Research Institute, University of California, San Francisco 94143.
J Appl Physiol (1985). 1994 Apr;76(4):1425-31. doi: 10.1152/jappl.1994.76.4.1425.
We compared surface tension measures of surfactants with various surface activities by using a pulsating bubble surfactometer (PBS) and a captive bubble surfactometer (CBS). Rabbit lung lavage surfactant (60,000 x average g for 60 min), bovine surfactant extract (Survanta), and a synthetic lipid surfactant mixture (dipalmitoylphosphatidylcholine-egg phosphatidylglycerol-palmitic acid) were studied at 1.25 mg phospholipid/ml. The PBS was used either unmodified according to manufacturer's instructions or with the sample chamber capillary kept dry and the sample adsorbing at maximum bubble size (5 min). The CBS was used in a manner that imitated the unmodified PBS. We found that all three techniques indicated low surface tension on the first cycle for 60K. For Survanta, the CBS and the modified PBS reported low surface tension on the first cycle, whereas the unmodified PBS did not achieve this within 10 cycles. For the synthetic lipid surfactant mixture, only the CBS measured low surface tension within 10 cycles. Video observations indicate that the modified PBS performs better than the unmodified PBS because keeping the capillary dry prevents surface film from occupying this large surface during cycling, thereby allowing larger area compressions.
我们使用脉动气泡表面张力仪（PBS）和俘获气泡表面张力仪（CBS）比较了具有不同表面活性的表面活性剂的表面张力测量值。研究了兔肺灌洗表面活性剂（60000倍平均重力，处理60分钟）、牛表面活性剂提取物（固尔苏）以及一种合成脂质表面活性剂混合物（二棕榈酰磷脂酰胆碱 - 鸡蛋磷脂酰甘油 - 棕榈酸），磷脂浓度为1.25mg/ml。PBS按照制造商说明未经修改使用，或者使样品室毛细管保持干燥，让样品在最大气泡尺寸时吸附（5分钟）。CBS以模仿未经修改的PBS的方式使用。我们发现，对于60K，所有三种技术在第一个循环时均显示低表面张力。对于固尔苏，CBS和经修改的PBS在第一个循环时报告了低表面张力，而未经修改的PBS在10个循环内未达到此值。对于合成脂质表面活性剂混合物，只有CBS在10个循环内测量到了低表面张力。视频观察表明，经修改的PBS比未经修改的PBS表现更好，因为保持毛细管干燥可防止表面膜在循环过程中占据这个大表面，从而允许更大面积的压缩。
