Putz G, Goerke J, Schürch S, Clements J A
Cardiovascular Research Institute, University of California, San Francisco 94143.
J Appl Physiol (1985). 1994 Apr;76(4):1417-24. doi: 10.1152/jappl.1994.76.4.1417.
We modified the captive bubble surfactometer [S. Schürch et al. J. Appl. Physiol. 67: 2389-2396, 1989] to facilitate the measurement of surface adsorption rates and to simplify its construction. We used a range of standards and monolayers of dipalmitoylphosphatidylcholine to check the calibration of the device against measurements made in a Wilhelmy surface balance and in the captive bubble by using a cathetometer, and we found good agreement. As a further test we measured the surface properties of rabbit lavage lung surfactant (60,000 x average g for 60 min) at 1.0 mg phospholipid/ml. This material adsorbed within 1 s to near-equilibrium surface tension, reached surface tensions of < 5 mN/m on the second compression, and formed very stable films. We conclude that a captive bubble surfactometer can provide accurate information about important surface properties of lung surfactant films.
我们对俘获气泡表面张力仪[S. 舒尔奇等人，《应用生理学杂志》67: 2389 - 2396, 1989]进行了改进，以利于测量表面吸附速率并简化其构造。我们使用了一系列标准品以及二棕榈酰磷脂酰胆碱单分子层，通过使用读数显微镜，对照在威尔海姆表面天平以及俘获气泡中所做的测量来检查该装置的校准情况，我们发现结果吻合良好。作为进一步的测试，我们在磷脂浓度为1.0毫克/毫升的条件下，测量了兔灌洗肺表面活性剂（60000倍平均重力，处理60分钟）的表面特性。这种物质在1秒内吸附至接近平衡表面张力，在第二次压缩时达到< 5毫牛顿/米的表面张力，并形成非常稳定的膜。我们得出结论，俘获气泡表面张力仪能够提供有关肺表面活性剂膜重要表面特性的准确信息。
