Pierres A, Benoliel A M, Bongrand P
Laboratoire d'Immunologie, INSERM U 387, Hôpital de Sainte-Marguerite, Marseille, France.
Cell Adhes Commun. 1998 Jul;5(5):375-95. doi: 10.3109/15419069809010783.
Cell adhesion is essentially mediated by specific interactions between membrane receptors and ligands. It is now apparent that the mere knowledge of the on- and off-rate of association of soluble forms of these receptors and ligands is not sufficient to yield accurate prediction of cell adhesive behavior. During the last few years, a variety of complementary techniques relying on the use of hydrodynamic flow, atomic force microscopy, surface forces apparatus or soft vesicles yielded accurate information on i) the dependence of the lifetime of individual bonds on applied forces and ii) the distance dependence of the association rate of bound receptors and ligands. The purpose of this review is, first to recall the physical significance of these parameters, and second to describe newly obtained results. It is emphasized that molecular size and flexibility may be a major determinant of the efficiency of receptor mediated adhesion, and this cannot be studied by conventional methods dealing with soluble molecules.
细胞黏附本质上是由膜受体与配体之间的特异性相互作用介导的。现在很明显，仅仅了解这些受体和配体的可溶性形式的结合和解离速率，并不足以准确预测细胞的黏附行为。在过去几年中，各种依赖于流体动力流、原子力显微镜、表面力装置或软囊泡的互补技术，提供了关于以下两方面的准确信息：i）单个键的寿命对所施加力的依赖性，以及ii）结合的受体和配体的缔合速率对距离的依赖性。本综述的目的，首先是回顾这些参数的物理意义，其次是描述新获得的结果。需要强调的是，分子大小和柔韧性可能是受体介导黏附效率的主要决定因素，而这无法通过处理可溶性分子的传统方法进行研究。
