Gunn-Moore D A, Gruffydd-Jones T J, Harbour D A
Department of Clinical Veterinary Science, University of Bristol, UK.
Vet Microbiol. 1998 Jul;62(3):193-205. doi: 10.1016/s0378-1135(98)00210-7.
A reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the detection of the feline coronavirus (FCoV) genome and a co-cultivation method for the isolation of field strains of FCoV are described. Using the RT-PCR assay to assess blood samples from cats with feline infectious peritonitis (FIP) (n = 47) and healthy cats from households with endemic FCoV (n = 69) it was shown that approximately 80% of the cats were viraemic, irrespective of their health status. It was also shown that, over a 12-month period, a similar percentage of healthy cats remained viraemic, and that the presence of viraemia did not appear to predispose the cats to the development of FIP. The co-cultivation system proved to be a suitable method for the culture of field strains of FCoV from blood samples, so long as the cultures were maintained for at least 4 weeks. Using this system, followed by the RT-PCR, viraemia was detected as frequently as by RT-PCR on RNA extracted directly from peripheral blood mononuclear cells.
本文描述了一种用于检测猫冠状病毒（FCoV）基因组的逆转录聚合酶链反应（RT-PCR）检测方法，以及一种用于分离FCoV野外毒株的共培养方法。使用RT-PCR检测方法评估患有猫传染性腹膜炎（FIP）的猫（n = 47）和来自FCoV流行家庭的健康猫（n = 69）的血液样本，结果显示约80%的猫存在病毒血症，无论其健康状况如何。研究还表明，在12个月的时间里，类似比例的健康猫仍存在病毒血症，且病毒血症的存在似乎并未使猫更容易患FIP。共培养系统被证明是一种从血液样本中培养FCoV野外毒株的合适方法，只要培养至少持续4周。使用该系统，随后进行RT-PCR，检测到病毒血症的频率与直接从外周血单核细胞提取的RNA上进行RT-PCR的频率相同。
