Freire-Santos F, Vergara-Castiblanco C A, Tojo-Rodriguez J L, Santamarina-Fernandez T, Ares-Mazas E
Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, La Coruña, España.
J Parasitol. 1998 Oct;84(5):935-8.
A study was carried out on rainbow trout Oncorhynchus mykiss fed with a commercial feed contaminated with bovine-isolated Cryptosporidium parvum oocysts whose viability and infectivity were previously tested by inoculation of oocysts to neonatal Swiss CD-1 mice. Histological examination of hematoxylin-eosin-stained gastrointestinal sections from control and C. parvum-exposed fish revealed no life-cycle stages of Cryptosporidium in any part of the apical border of the digestive tract. However, sections of the stomach and pyloric region from exposed fish displayed large numbers of 5-7-microm spherical structures located deep within the epithelial tissue. Under conditions of host stress, the number of these structures increased remarkably. An immunofluorescence antibody test with IgG and IgM anti-cryptosporidial antibodies revealed fluorescence reactivity in these structures. Simultaneously, wild trout were analyzed in order to detect natural cryptosporidial infections; Cryptosporidium oocyst-like bodies were found in the intestinal content of 10% of the specimens.
对用受牛源微小隐孢子虫卵囊污染的商业饲料喂养的虹鳟（Oncorhynchus mykiss）进行了一项研究，这些卵囊的活力和感染性先前已通过将卵囊接种到新生瑞士CD - 1小鼠身上进行了测试。对来自对照鱼和暴露于微小隐孢子虫的鱼的苏木精 - 伊红染色胃肠道切片进行组织学检查，结果显示在消化道顶端边界的任何部位均未发现隐孢子虫的生命周期阶段。然而，暴露鱼的胃和幽门区域切片显示上皮组织深处有大量直径为5 - 7微米的球形结构。在宿主应激条件下，这些结构的数量显著增加。用抗隐孢子虫IgG和IgM抗体进行的免疫荧光抗体试验显示这些结构中有荧光反应性。同时，对野生鳟鱼进行分析以检测自然隐孢子虫感染；在10%的样本肠道内容物中发现了隐孢子虫样卵囊体。
