Velert M M, Cabrera E, Orero A, Pemán J, Cantón E
Unidad de Bacteriología Experimental, Centro de Investigación.
Rev Esp Quimioter. 1998 Mar;11(1):70-4.
The effect of pretreatment of human polymorphonuclear leukocites (PMNs), for 30 min with fluconazole (0.1, 1, 5 and 50 microgram/ml) and itraconazole (0.05, 0.5 and 5 microgram/ml) on phagocytosis and generation of free radicals (superoxide anion and hydrogen peroxide) was studied in vitro. Phorbol miristate acetate (200 nM) was used as a stimulant. The mean amount of superoxide anion generated by 2.5 x 10(5) PMNs per hour was 4.39 +/- 1.13 nmol for fluconazole-treated PMNs and 4.56 +/- 1.2 nmol for itraconazole, and that of hydrogen peroxide was 11.19 +/- 2.18 and 11.28 +/- 3.61 nmol, respectively. The phagocytosis percentages were 83.8% for the control group and 88. 7% in antifungal agent- treated PMNs; the phagocytosis index was 3.0 yeasts per PMN for both groups. The differences between the control and treated PMNs were not statistically significant at any of the tested concentrations.
在体外研究了用氟康唑（0.1、1、5和50微克/毫升）和伊曲康唑（0.05、0.5和5微克/毫升）对人多形核白细胞（PMN）进行30分钟预处理后，其对吞噬作用和自由基（超氧阴离子和过氧化氢）生成的影响。佛波酯（200纳摩尔）用作刺激剂。每小时2.5×10⁵个PMN产生的超氧阴离子平均量，氟康唑处理的PMN为4.39±1.13纳摩尔，伊曲康唑处理的PMN为4.56±1.2纳摩尔，过氧化氢的平均量分别为11.19±2.18纳摩尔和11.28±3.61纳摩尔。对照组的吞噬百分比为83.8%，抗真菌剂处理的PMN为88.7%；两组的吞噬指数均为每个PMN吞噬3.0个酵母。在任何测试浓度下，对照组和处理后的PMN之间的差异均无统计学意义。
