Lagatolla C, Lavenia A, Tonin E, Monti-Bragadin C, Dolzani L
Dipartimento di Scienze Biomediche, Università degli Studi, Trieste, Italy.
Res Microbiol. 1998 Sep;149(8):557-66. doi: 10.1016/s0923-2508(99)80003-5.
The 16S-23S intergenic spacer regions of four Acinetobacter genomic species belonging to the A. calcoaceticus-A. baumannii (Acb) complex, i.e. genomic species 1 (A. calcoaceticus), genomic species 2 (A. baumannii), genomic species 3 and Tjernberg and Ursing (TU) genomic species 13, have been cloned and sequenced. Sequence analysis led to the discovery of a single copy of IIe and Ala tRNA genes within each spacer. Sequence comparison allowed the identification of a 192-base-pair long highly conserved sequence between the 3' end of the 16S rRNA and the 5' end of the tRNA(Ala) genes. Moreover, two short regions, which were specific to, respectively, genomic species 2 and 3, could be identified. Oligonucleotides corresponding to these sequences were constructed and tested for the ability to hybridize with chromosomal DNA extracted from Acinetobacter belonging to different genomic species and with chromosomal DNA of other bacterial genera. One of these oligonucleotides was demonstrated to be useful as a sensitive and specific probe for A. baumannii. A less sensitive probe for Acinetobacter genomic species 3 was also developed.
属于醋酸钙不动杆菌-鲍曼不动杆菌（Acb）复合体的4个不动杆菌基因组种，即基因组种1（醋酸钙不动杆菌）、基因组种2（鲍曼不动杆菌）、基因组种3和Tjernberg与Ursing（TU）基因组种13的16S-23S基因间隔区已被克隆和测序。序列分析发现每个间隔区内有单个拷贝的异亮氨酸和丙氨酸tRNA基因。序列比较确定了16S rRNA 3'端与tRNA（Ala）基因5'端之间一段192个碱基对长的高度保守序列。此外，还可鉴定出分别对基因组种2和3特异的两个短区域。构建了与这些序列对应的寡核苷酸，并测试了它们与从属于不同基因组种的不动杆菌提取的染色体DNA以及其他细菌属的染色体DNA杂交的能力。其中一个寡核苷酸被证明可作为鲍曼不动杆菌的灵敏且特异的探针。还开发了一种对不动杆菌基因组种3不太灵敏的探针。
