Thornton M H, Francis M M, Paulson R J
Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles, USA.
Fertil Steril. 1998 Oct;70(4):647-50. doi: 10.1016/s0015-0282(98)00274-x.
To describe retrieval of immature oocytes during unstimulated IVF and assess the in vitro maturation and fertilization rates.
Retrospective analysis.
The USC program for assisted reproduction.
PATIENT(S): Spontaneously ovulatory women with predominantly pelvic factor as their principal cause of infertility, under the age of 40, and no male factor.
INTERVENTION(S): HCG administration in mid-cycle, aspiration of all visible follicles, in vitro maturation of immature oocytes in culture media versus 50% follicular fluid in media, IVF, and embryo transfer.
MAIN OUTCOME MEASURE(S): Rates of in vitro maturation, fertilization, and implantation after embryo transfer.
RESULT(S): A total of 101 immature oocytes were obtained during 59 follicle aspirations. Thirty percent of prophase I oocytes matured to metaphase II in vitro compared with 44% of metaphase I oocytes. Fertilization rates for matured prophase I oocytes were 62% for those cultured in standard culture media (controls) and 87% for follicular fluid culture media. Two pregnancies resulted from the transfer of embryos derived from immature oocytes when no other embryos were transferred.
CONCLUSION(S): Immature oocytes may be retrieved successfully during the mid-cycle aspiration of the dominant follicle in unstimulated IVF cycles. Maturation of immature oocytes in vitro with follicular fluid results in similar maturation and fertilization rates as for control incubation. Immature oocytes thus retrieved contribute to the overall pregnancy success of unstimulated IVF cycles. It may be better to retrieve immature oocytes during unstimulated cycles than during the follicular phase of natural cycles.
描述在未刺激的体外受精过程中未成熟卵母细胞的获取情况，并评估其体外成熟率和受精率。
回顾性分析。
南加州大学辅助生殖项目。
以盆腔因素为主因导致不孕、年龄在40岁以下且无男性因素的自发排卵女性。
在月经周期中期注射人绒毛膜促性腺激素（HCG），抽吸所有可见卵泡，将未成熟卵母细胞在培养基与含50%卵泡液的培养基中进行体外成熟培养，进行体外受精（IVF）及胚胎移植。
胚胎移植后的体外成熟率、受精率和着床率。
在59次卵泡抽吸过程中共获取101个未成熟卵母细胞。与44%的中期I卵母细胞相比，30%的前期I卵母细胞在体外成熟至中期II。在标准培养基（对照组）中培养的成熟前期I卵母细胞的受精率为62%，在卵泡液培养基中培养的受精率为87%。当未移植其他胚胎时，由未成熟卵母细胞来源的胚胎移植导致了2例妊娠。
在未刺激的体外受精周期中，于月经周期中期抽吸优势卵泡时可成功获取未成熟卵母细胞。用卵泡液对未成熟卵母细胞进行体外成熟培养，其成熟率和受精率与对照培养相似。如此获取的未成熟卵母细胞有助于未刺激的体外受精周期的整体妊娠成功率。在未刺激周期中获取未成熟卵母细胞可能比在自然周期的卵泡期获取更好。
