植物和动物半胱氨酸蛋白酶抑制剂之间的结构和系统发育关系。
Structural and phylogenetic relationships among plant and animal cystatins.
作者信息
Margis R, Reis E M, Villeret V
机构信息
Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil.
出版信息
Arch Biochem Biophys. 1998 Nov 1;359(1):24-30. doi: 10.1006/abbi.1998.0875.
The plant cystatins or phytocystatins (PhyCys) are cysteine proteinase inhibitors containing the QxVxG motif and have been placed in the cystatin superfamily of proteins. The primary sequences of PhyCys have a high degree of homology with the members of the cystatin family, but they resemble stefins by the absence of disulfide bonds and cysteine residues. A multialignment and a phylogenetic analysis of 63 cystatins, 32 of which are PhyCys, demonstrate that all PhyCys cluster in a major evolutionary tree branch and support the classification of PhyCys as a new cystatin family. The PhyCys also possess a specific consensus sequence [LVI]-[AGT]-[RKE]-[FY]-[AS]-[VI]-x-[EDQV]-[HYFQ] -N placed on the region corresponding to a predictable amino-terminal alpha-helix. This sequence can be used to specifically identify PhyCys on protein data banks and to differentiate them from the other members of the superfamily.
植物半胱氨酸蛋白酶抑制剂（PhyCys）含有QxVxG基序，属于蛋白质半胱氨酸蛋白酶抑制剂超家族。PhyCys的一级序列与半胱氨酸蛋白酶抑制剂家族成员具有高度同源性，但由于缺乏二硫键和半胱氨酸残基，它们与丝抑蛋白相似。对63种半胱氨酸蛋白酶抑制剂（其中32种为PhyCys）进行的多序列比对和系统发育分析表明，所有PhyCys都聚集在一个主要的进化树分支中，支持将PhyCys归类为一个新的半胱氨酸蛋白酶抑制剂家族。PhyCys在对应于可预测的氨基末端α-螺旋的区域还具有一个特定的共有序列[LVI]-[AGT]-[RKE]-[FY]-[AS]-[VI]-x-[EDQV]-[HYFQ]-N。该序列可用于在蛋白质数据库中特异性鉴定PhyCys，并将它们与超家族的其他成员区分开来。
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