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麦芽糖酶的热失活及其在酵母温度敏感突变体中的应用。

Thermal inactivation of maltase and its application to temperature-sensitive mutants of yeast.

作者信息

Eaton N R, Zimmermann F K

出版信息

Mol Gen Genet. 1976 Oct 18;148(2):199-204. doi: 10.1007/BF00268385.

DOI:10.1007/BF00268385
PMID:979959
Abstract

Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, lack the maltase of intermediate stability. This deficiency appears to be the basis of the temperature sensitivity, and it is concluded that these strains carry a mutation in the structural gene for that maltase.

摘要

在多种酿酒酵母菌株中,每种都检测到了三种麦芽糖酶。这些麦芽糖酶在特定菌株中以恒定的相对含量存在,并具有可重复的一级热衰变常数。六个对麦芽糖发酵温度敏感的突变体中有四个缺乏稳定性中等的麦芽糖酶。这种缺陷似乎是温度敏感性的基础,由此得出结论,这些菌株在该麦芽糖酶的结构基因中发生了突变。

相似文献

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引用本文的文献

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The dispersed, repeated family of MAL loci in Saccharomyces spp.

本文引用的文献

1
Genetic control of maltase formation in yeast. III. Isolation and characterization of temperature-sensitive mutants affecting maltase induction and maltose utilization.
Mol Gen Genet. 1975;136(1):55-61. doi: 10.1007/BF00275448.
2
Comparison of the alpha-glucosidases of Saccharomyces produced in response to five non-allelic maltose genes.酿酒酵母响应五个非等位麦芽糖基因所产生的α-葡萄糖苷酶的比较。
Biochim Biophys Acta. 1963 Jan 8;67:42-53. doi: 10.1016/0006-3002(63)91795-5.
3
The purification and properties of an alpha-glucosidase of Saccharomyces italicus Y1225.意大利酵母Y1225的α-葡萄糖苷酶的纯化及性质
酿酒酵母属中MAL基因座的分散重复家族
J Bacteriol. 1984 Mar;157(3):949-52. doi: 10.1128/jb.157.3.949-952.1984.
Biochim Biophys Acta. 1958 Oct;30(1):28-40. doi: 10.1016/0006-3002(58)90237-3.
4
Regulation of maltose fermentation in Saccharomyces carlsbergensis. I. The function of the gene MAL6, as recognized by mal6-mutants.卡尔斯伯酵母中麦芽糖发酵的调控。I. 由mal6突变体所识别的MAL6基因的功能。
Mol Gen Genet. 1973 Jul 2;123(3):233-46. doi: 10.1007/BF00271242.
5
Genetics of induction and catabolite repression of Maltese synthesis in Saccharomyces cerevisiae.酿酒酵母中麦芽糖合成的诱导与分解代谢阻遏的遗传学
Mol Gen Genet. 1974;134(3):261-72. doi: 10.1007/BF00267720.
6
Genetic control of maltase formation in yeast. II. Evidence for a gene regulating the level of maltase production.
Mol Gen Genet. 1973 Aug 28;124(4):365-7. doi: 10.1007/BF00267665.
7
Induction, selection, and experimental uses of temperature-sensitive and other conditional mutants of yeast.
Methods Cell Biol. 1975;12:233-72. doi: 10.1016/s0091-679x(08)60959-0.