Touitou E, Meidan V M, Horwitz E
Department of Pharmaceutics, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 91120, Israel.
J Control Release. 1998 Dec 4;56(1-3):7-21. doi: 10.1016/s0168-3659(98)00060-1.
The quantification of drugs within the skin is essential for topical and transdermal delivery research. Over the last two decades, horizontal sectioning, consisting of both tape stripping and parallel slicing through the deeper tissues has constituted the traditional investigative technique. In recent years, this methodology has been augmented by such procedures as heat separation, qualitative autoradiography, isolation of the pilosebaceous units and the use of induced follicle-free skin. The development of skin quantitative autoradiography represents an entirely novel approach which permits quantification and visualization of the penetrant throughout a vertical cross-section of skin. Noninvasive strategies involve the application of optical measuring systems such as attenuated total reflectance Fourier transform infrared, fluorescence, remittance or photothermal spectroscopies.
皮肤内药物的定量分析对于局部和透皮给药研究至关重要。在过去二十年中，水平切片，包括胶带剥离和对更深层组织进行平行切片，一直是传统的研究技术。近年来，这种方法已通过热分离、定性放射自显影、毛囊皮脂腺单位分离以及使用诱导无毛囊皮肤等程序得到补充。皮肤定量放射自显影的发展代表了一种全新的方法，它能够在皮肤的垂直横截面中对渗透剂进行定量和可视化。非侵入性策略涉及应用光学测量系统，如衰减全反射傅里叶变换红外光谱、荧光、反射或光热光谱。
