Huang C N, Tsai J L, Chen M T, Wu W J, Kuo K W, Huang C H
Department of Urology, Kaohsiung Medical College, Taiwan.
Biochem Mol Biol Int. 1998 Oct;46(2):375-83. doi: 10.1080/15216549800203882.
To investigate changes in the activities of mitochondrial enzymes in the progress of tumorigenesis of bladder cancer, N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) carcinogen was administered orally to male Sprague-Dawley rats for eight weeks. The urinary bladders were harvested periodically for the evaluation of tumorigenesis. The activities of NADH cytochrome c reductase (NCCR), succinate cytochrone c reductase (SCCR) and cytochrome c oxidase (CCO) were measured. The NCCR and SCCR activities elevated significantly by the exposure of BBN and decreased rapidly when BBN was withdrawn. However, the CCO activity increased and reached plateau at 18 weeks in spite of the discontinuance of BBN. The results indicated that the NCCR, SCCR and CCO activities were significantly elevated in the tumorigenesis. However, the CCO enzyme may be more related to the progress of tumorigenesis of bladder cancer.
为研究膀胱癌发生过程中线粒体酶活性的变化，对雄性Sprague-Dawley大鼠口服N-丁基-N-(4-羟丁基)亚硝胺（BBN）致癌物8周。定期采集膀胱以评估肿瘤发生情况。测量了NADH细胞色素c还原酶（NCCR）、琥珀酸细胞色素c还原酶（SCCR）和细胞色素c氧化酶（CCO）的活性。BBN暴露使NCCR和SCCR活性显著升高，撤去BBN后迅速下降。然而，尽管停止给予BBN，CCO活性仍在18周时升高并达到平台期。结果表明，在肿瘤发生过程中NCCR、SCCR和CCO活性显著升高。然而，CCO酶可能与膀胱癌肿瘤发生的进展更相关。
