Srikantha Thyagarajan, Tsai Luong, Daniels Karla, Enger Lee, Highley Kumiko, Soll David R
Microbiology (Reading). 1998 Oct;144 ( Pt 10):2715-2729. doi: 10.1099/00221287-144-10-2715.
Using degenerate primers of highly conserved regions of two-component response regulators for PCR amplification, a two-component response regulator was cloned from Candida albicans that is homologous to nik-1+ of Neurospora crassa. This two-component hybrid kinase, CaNIK1, also shows features of bacterial two-component response regulators, including a putative unorthodox second histidine kinase motif at the carboxy-terminal end. CaNIK1 was expressed at low levels in both the white and opaque switch phenotypes and in the bud and hyphal growth forms of C. albicans strain WO-1, but in both developmental programmes, the level of transcript was modulated (levels were higher in opaque cells and in hyphae). Partial deletion of both CaNIK1 alleles, by which the histidine autokinase- and ATP-binding domains were removed, did not inhibit either high-frequency phenotypic switching or the bud-hypha transition in high salt concentrations, but in both cases the efficiency of the developmental process was reduced.
利用双组分反应调节因子高度保守区域的简并引物进行聚合酶链反应（PCR）扩增，从白色念珠菌中克隆出一种双组分反应调节因子，它与粗糙脉孢菌的nik-1+同源。这种双组分杂交激酶CaNIK1也具有细菌双组分反应调节因子的特征，包括在羧基末端有一个假定的非传统第二组氨酸激酶基序。CaNIK1在白色念珠菌菌株WO-1的白色和不透明转换表型以及芽和菌丝生长形式中均低水平表达，但在这两个发育程序中，转录水平受到调节（不透明细胞和菌丝中的水平较高）。通过去除组氨酸自激酶和ATP结合结构域对CaNIK1的两个等位基因进行部分缺失，并不抑制高盐浓度下的高频表型转换或芽-菌丝转变，但在这两种情况下，发育过程的效率均降低。
