Lilja M, Räisänen S, Stenfors L E
Department of Otolaryngology, University of Tromsø, Norway.
Int J Pediatr Otorhinolaryngol. 1998 Sep 15;45(1):15-20. doi: 10.1016/s0165-5876(98)00071-8.
Bacterial and epithelial cell samples were obtained, within 24 h of onset of pharyngeal symptoms, from the palatine tonsils of nine patients (four female and five male; age range 10-40 years, median age 23) with acute tonsillitis, culture-positive for Streptococcus pyogenes. The specimens were examined using fluorescein isothiocyanate- (FITC) and gold-labelled antiserum to S. pyogenes and fluorescence, scanning electron and transmission electron microscopy. S. pyogenes could be identified both in the mucous layer covering the tonsils and attached to the surface epithelial cells. Long chains of coccus-shaped bacteria could be seen encroaching on the epithelial cell borders. S. pyogenes can apparently penetrate the mucous barrier, attach to the epithelial cells, spread from cell to cell and possibly penetrate into the outermost layer of the epithelial cells. These events in turn provoke cytokine production and/or complement activation, which induce inflammatory reaction in the tonsillar tissue.
在出现咽部症状的24小时内，从9例急性扁桃体炎患者（4名女性和5名男性；年龄范围10 - 40岁，中位年龄23岁）的腭扁桃体获取细菌和上皮细胞样本，这些患者的化脓性链球菌培养呈阳性。使用异硫氰酸荧光素（FITC）和抗化脓性链球菌的金标记抗血清以及荧光、扫描电子和透射电子显微镜对标本进行检查。在覆盖扁桃体的黏液层以及附着于表面上皮细胞上均能识别出化脓性链球菌。可以看到球菌形状的细菌长链侵入上皮细胞边界。化脓性链球菌显然能够穿透黏液屏障，附着于上皮细胞，在细胞间传播，并可能穿透到上皮细胞的最外层。这些情况进而引发细胞因子产生和/或补体激活，从而在扁桃体组织中诱导炎症反应。
