Vigreux C, Poul J M, Deslandes E, Lebailly P, Godard T, Sichel F, Henry-Amar M, Gauduchon P
INSERM CJF 96-03 and EA 1772, Laboratoire de Cancérologie Expérimentale, Centre François Baclesse, Route de Lion-sur-mer, 14076, Caen Cedex 05, France.
Mutat Res. 1998 Nov 9;419(1-3):79-90. doi: 10.1016/s1383-5718(98)00126-0.
One herbicide (isoproturon), two fungicides (carbendazim and chlorothalonil) and etoposide (an effective antitumor agent used as a positive control), were tested for their ability to induce cytotoxic and genotoxic effects in Chinese Hamster Ovary (CHOK1) cells. Etoposide induced DNA damage detectable both by the alkaline Single Cell Gel Electrophoresis (SCGE) assay and the chromosomal aberration (CA) test in absence of noticeable cytotoxicity. With the SCGE assay, a clear induction of DNA damage was observed for chlorothalonil within a 0.2 to 1 microM concentration range. In the CA test, chlorothalonil gave also positive results, inducing mainly chromosome breaks. In contrast, no DNA damage was observed with the SCGE assay for carbendazim and isoproturon. In the CA test, carbendazim induced only numerical aberrations in the concentration range of 25 microM to 100 microM, and isoproturon did not induce any significant increase in CA. In conclusion, chlorothalonil appears genotoxic in proliferative CHOK1 cells, and as expected, the aneugenic compound, carbendazim, did not induce DNA strand breaks in the SCGE assay.
对一种除草剂（异丙隆）、两种杀菌剂（多菌灵和百菌清）以及依托泊苷（一种用作阳性对照的有效抗肿瘤剂）进行了测试，以检测它们在中国仓鼠卵巢（CHOK1）细胞中诱导细胞毒性和遗传毒性效应的能力。依托泊苷在未产生明显细胞毒性的情况下，通过碱性单细胞凝胶电泳（SCGE）试验和染色体畸变（CA）试验均可检测到其诱导的DNA损伤。使用SCGE试验时，在0.2至1微摩尔浓度范围内，可观察到百菌清对DNA损伤有明显诱导作用。在CA试验中，百菌清也给出了阳性结果，主要诱导染色体断裂。相比之下，使用SCGE试验未观察到多菌灵和异丙隆对DNA的损伤。在CA试验中，多菌灵仅在25微摩尔至100微摩尔浓度范围内诱导了数量畸变，而异丙隆未诱导CA有任何显著增加。总之，百菌清在增殖性CHOK1细胞中表现出遗传毒性，并且正如预期的那样，非整倍体化合物多菌灵在SCGE试验中未诱导DNA链断裂。
