Hisatomi T, Wada Y, Fujisaki C, Tsuboi M
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.
Curr Microbiol. 1998 Dec;37(6):426-30. doi: 10.1007/s002849900404.
We examined the autonomously replicating sequence (ARS) activity of some fragments derived from the LEU2 region of Saccharomyces cerevisiae onto Saccharomyces exiguus Yp74L-3. A DNA fragment functioning as an ARS in S. exiguus, but not in S. cerevisiae, was shown to exist. The ARS activity for S. exiguus was reduced by the 2-microm plasmid origin of S. cerevisiae when both elements coexisted on a single circular plasmid. Analysis of ARS activity with the PCR products from the fragment revealed that the ARS-acting sequence was located in the 3'-terminal area of the transcribed region of the LEU2 gene of S. cerevisiae. It is suggested that the ARS recognition system in S. exiguus is significantly different from that of S. cerevisiae.
我们检测了从酿酒酵母LEU2区域衍生的一些片段在嗜杀酵母Yp74L-3上的自主复制序列（ARS）活性。结果显示，存在一个在嗜杀酵母中起ARS作用但在酿酒酵母中不起作用的DNA片段。当这两种元件共存于单个环状质粒上时，酿酒酵母的2-μm质粒原点会降低嗜杀酵母的ARS活性。对该片段PCR产物的ARS活性分析表明，ARS作用序列位于酿酒酵母LEU2基因转录区域的3'末端区域。这表明嗜杀酵母中的ARS识别系统与酿酒酵母的显著不同。
