Hisatomi T, Kubota K, Tsuboi M
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.
Curr Microbiol. 1999 Feb;38(2):122-5. doi: 10.1007/s002849900414.
Fragments containing ARSes were cloned from the genomic DNA of the yeast Saccharomyces exiguus Yp74L-3, and the essential regions for ARSes were restricted for these fragments. Mapping studies of ARS-acting sequences in one of these fragments suggested that S. exiguus recognizes a sequence as an ARS that is different from that recognized by Saccharomyces cerevisiae. Two ARS essential regions of S. exiguus were sequenced, and an ARS core consensus sequence of S. exiguus was deduced to be MATTAMWAWWTK. This sequence differs significantly from that of S. cerevisiae in two positions, suggesting that these nucleotide substitutions cause the difference in the ARS-recognition modes between S. exiguus and S. cerevisiae.
从酵母嗜杀酵母Yp74L-3的基因组DNA中克隆出含有自主复制序列（ARS）的片段，并对这些片段中ARS的必需区域进行了限制。对其中一个片段中的ARS作用序列进行的定位研究表明，嗜杀酵母识别的作为ARS的序列与酿酒酵母识别的序列不同。对嗜杀酵母的两个ARS必需区域进行了测序，推断出嗜杀酵母的ARS核心共有序列为MATTAMWAWWTK。该序列在两个位置上与酿酒酵母的序列有显著差异，表明这些核苷酸取代导致了嗜杀酵母和酿酒酵母在ARS识别模式上的差异。
