Wakimoto M, Masuoka N, Nakano T, Ubuka T
Department of Biochemistry, Okayama University Medical School, Japan.
Acta Med Okayama. 1998 Oct;52(5):233-7. doi: 10.18926/AMO/31323.
A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.
开发了一种测定红细胞中谷胱甘肽过氧化物酶活性的新方法。本方法用于在模拟体内条件下，测定红细胞中谷胱甘肽过氧化物酶在70微摩尔过氧化氢存在时的过氧化氢清除率。小鼠红细胞中谷胱甘肽过氧化物酶的清除率比人类红细胞快20倍，为5.2微摩尔/秒/克血红蛋白。无过氧化氢酶小鼠红细胞中的清除率表明，谷胱甘肽过氧化物酶是无过氧化氢酶小鼠红细胞中过氧化氢清除的主要方式。基于这些结果，我们得出结论，小鼠红细胞中的谷胱甘肽过氧化物酶即使在相对高浓度下也有足够的能力清除过氧化氢。这可能是无过氧化氢酶小鼠没有健康问题，而日本无过氧化氢酶患者在感染产生过氧化氢的细菌时会患原田病的原因之一。
