Sagara N, Toda G, Hirai M, Terada M, Katoh M
Genetics Division, National Cancer Center Research Institute, Tsukiji 5-chome, Chuo-ku, Tokyo, 104-0045, Japan.
Biochem Biophys Res Commun. 1998 Nov 9;252(1):117-22. doi: 10.1006/bbrc.1998.9607.
cDNAs for three human Wnt receptors, Frizzled-1 (FZD1), Frizzled-2 (FZD2), and Frizzled-7 (FZD7), have now been cloned and characterized. The FZD1, FZD2, and FZD7 genes encode the 647-, 565-, and 574-amino-acid proteins, respectively. FZD1, FZD2, and FZD7 share a common structure consisting of seven transmembrane domains, a cysteine-rich domain in the N-terminal extracellular region, and the C-terminal Ser/Thr-Xxx-Val motif. Relatively large amounts of FZD 1 mRNA, 4.5 kb in size, were detected in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. FZD2 mRNAs 4.0 and 2.4 kb in size were detected in adult heart, fetal brain, lung, and kidney. The level of FZD7 mRNAs 5.0 and 4.0 kb in size was high in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. The FZD1 and FZD7 genes have been mapped to human chromosome 7q21 and 2q33, respectively.
现已克隆并鉴定了三种人类Wnt受体的互补DNA（cDNA），即卷曲蛋白-1（FZD1）、卷曲蛋白-2（FZD2）和卷曲蛋白-7（FZD7）。FZD1、FZD2和FZD7基因分别编码含647、565和574个氨基酸的蛋白质。FZD1、FZD2和FZD7具有共同的结构，由七个跨膜结构域、N端细胞外区域富含半胱氨酸的结构域以及C端的丝氨酸/苏氨酸-XXX-缬氨酸基序组成。在成人心脏、胎盘、肺、肾、胰腺、前列腺和卵巢以及胎儿肺和肾中检测到相对大量大小为4.5 kb的FZD1 mRNA。在成人心脏、胎儿脑、肺和肾中检测到大小分别为4.0和2.4 kb的FZD2 mRNA。在成人骨骼肌和胎儿肾中，大小为5.0和4.0 kb的FZD7 mRNA水平较高，其次是胎儿肺、成人心脏、脑和胎盘。FZD1和FZD7基因已分别定位于人类染色体7q21和2q33。
