Tokuhara M, Hirai M, Atomi Y, Terada M, Katoh M
Genetics Division, National Cancer Center Research Institute Tsukiji 5-chome, Tokyo, Japan.
Biochem Biophys Res Commun. 1998 Feb 13;243(2):622-7. doi: 10.1006/bbrc.1998.8143.
The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in the N-terminal extracellular region, two N-linked glycosylation sites, and two cystein residues in the second and third extracellular loops. Hfz6 mRNA 4.4-kb in size was detected in various normal adult and fetal tissues, and a larger amount of Hfz6 mRNA was detected in both fetal lung and fetal kidney. The Hfz6 gene has been mapped to human chromosome 8q22.3-q23.1. In conclusion, we have cloned Hfz6, which encodes a seven-transmembrane receptor with the cystein-rich domain in the N-terminal extracellular region, but without the Ser/Thr-X-Val motif in the C-terminus.
卷曲蛋白基因编码WNTs的受体，WNTs是一种分泌型糖蛋白，与发育以及致癌作用有关。在本文中，我们报告了Mfz6的人类同源物Hfz6的分子克隆。核苷酸序列分析表明，Hfz6基因编码具有七个跨膜结构域的706个氨基酸的蛋白质，在N端细胞外区域有一个富含半胱氨酸的结构域，两个N-连接糖基化位点，以及在第二和第三细胞外环中有两个半胱氨酸残基。在各种正常成人和胎儿组织中检测到大小为4.4kb的Hfz6 mRNA，并且在胎儿肺和胎儿肾中均检测到大量的Hfz6 mRNA。Hfz6基因已被定位到人类染色体8q22.3-q23.1。总之，我们克隆了Hfz6，它编码一种在N端细胞外区域具有富含半胱氨酸结构域的七跨膜受体，但在C端没有Ser/Thr-X-Val基序。
