Maillet S, Vion-Dury J, Confort-Gouny S, Nicoli F, Lutz N W, Viout P, Cozzone P J
Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine, 27 Bd Jean Moulin, F-13005, Marseille, France.
Brain Res Brain Res Protoc. 1998 Nov;3(2):123-34. doi: 10.1016/s1385-299x(98)00033-6.
High resolution magnetic resonance spectroscopy (MRS) of cerebrospinal fluid (CSF) is a non-destructive analytical method which allows rapid and simultaneous detection of molecules involved in intermediary and oxidative metabolic pathways. We developed a protocol suitable for routine MRS analysis of lyophilized CSF samples. This procedure guarantees sample integrity, from CSF collection to spectrum acquisition. MRS analysis of blood serum was included in our protocol as a complementary method to CSF analysis. This protocol can contribute to establish MRS of CSF as a new analytical tool to better understand the metabolic processes involved in neurological diseases.
脑脊液（CSF）的高分辨率磁共振波谱（MRS）是一种非破坏性分析方法，可快速同时检测参与中间代谢和氧化代谢途径的分子。我们开发了一种适用于冻干脑脊液样本常规MRS分析的方案。该程序可确保从脑脊液采集到光谱采集的样本完整性。血清的MRS分析作为脑脊液分析的补充方法包含在我们的方案中。该方案有助于将脑脊液MRS确立为一种新的分析工具，以更好地理解神经疾病中涉及的代谢过程。
