Musteata Mihai, Nicolescu Alina, Solcan Gheorghe, Deleanu Calin
Clinics Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Iasi, Romania.
Group of Biospectroscopy, Institute of Macromolecular Chemistry, Roumanian Academy, Iasi, Romania ; Group of Biospectroscopy, Centre of Organic Chemistry, Roumanian Academy, Bucharest, Romania.
PLoS One. 2013 Dec 23;8(12):e81192. doi: 10.1371/journal.pone.0081192. eCollection 2013.
The availability of data for reference values in cerebrospinal fluid for healthy humans is limited due to obvious practical and ethical issues. The variability of reported values for metabolites in human cerebrospinal fluid is quite large. Dogs present great similarities with humans, including in cases of central nervous system pathologies. The paper presents the first study on healthy dog cerebrospinal fluid metabolomic profile using (1)H NMR spectroscopy. A number of 13 metabolites have been identified and quantified from cerebrospinal fluid collected from a group of 10 mix breed healthy dogs. The biological variability as resulting from the relative standard deviation of the physiological concentrations of the identified metabolites had a mean of 18.20% (range between 9.3% and 44.8%). The reported concentrations for metabolites may be used as normal reference values. The homogeneity of the obtained results and the low biologic variability show that the (1)H NMR analysis of the dog's cerebrospinal fluid is reliable in designing and interpreting clinical and therapeutic trials in dogs with central nervous system pathologies.
由于明显的实际和伦理问题，健康人类脑脊液参考值的数据可用性有限。人类脑脊液中代谢物报告值的变异性相当大。狗与人类有很多相似之处，包括在中枢神经系统病理学方面。本文首次利用氢核磁共振波谱对健康犬脑脊液代谢组学特征进行了研究。从一组10只健康混种犬采集的脑脊液中鉴定并定量了13种代谢物。所鉴定代谢物生理浓度的相对标准偏差导致的生物学变异性平均为18.20%（范围在9.3%至44.8%之间）。所报告的代谢物浓度可用作正常参考值。所得结果的同质性和低生物学变异性表明，犬脑脊液的氢核磁共振分析在设计和解释患有中枢神经系统疾病犬的临床和治疗试验中是可靠的。
