Puren A J, Razeghi P, Fantuzzi G, Dinarello C A
Department of Medicine, Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, USA.
J Infect Dis. 1998 Dec;178(6):1830-4. doi: 10.1086/314481.
Interleukin-18 (IL-18) is a newly described cytokine, formerly called interferon-gamma (IFN-gamma)-inducing factor. In a simple 24-h human whole blood culture, IFN-gamma was produced by the combination of lipopolysaccharide (LPS) plus IL-18. To liberate cytokines in the leukocyte and red cell compartments, the detergent Triton X-100 was added to the entire blood culture. The combination of low concentrations of LPS plus IL-18 induced a 3- to 5-fold greater production of IFN-gamma than did either stimulant alone. Tumor necrosis factor-alpha (TNF-alpha), IL-6, and IL-8 were also produced. The presence of IL-10 completely suppressed the production of IFN-gamma and reduced that of TNF-alpha, IL-6, and IL-8. Thus, IFN-gamma, TNF-alpha, IL-8, and IL-6 are produced in a single whole blood culture, making correlations in the synthesis of a T helper type 1 cytokine and proinflammatory cytokines with disease activity possible in a single culture.
白细胞介素-18（IL-18）是一种新发现的细胞因子，以前称为干扰素-γ（IFN-γ）诱导因子。在简单的24小时人体全血培养中，脂多糖（LPS）与IL-18联合可产生IFN-γ。为了释放白细胞和红细胞中的细胞因子，将去污剂曲拉通X-100添加到全血培养物中。低浓度LPS与IL-18联合诱导产生的IFN-γ比单独使用任何一种刺激剂时高3至5倍。还产生了肿瘤坏死因子-α（TNF-α）、IL-6和IL-8。IL-10的存在完全抑制了IFN-γ的产生，并降低了TNF-α、IL-6和IL-8的产生。因此，在单一全血培养中可产生IFN-γ、TNF-α、IL-8和IL-6，这使得在单一培养中研究1型辅助性T细胞因子与促炎细胞因子合成与疾病活动之间的相关性成为可能。
