Nam K H, Akari H, Terao K, Itagaki S, Yoshikawa Y
Tsukuba Primate Center, National Institute of Infectious Diseases, Ibaraki, Japan.
Exp Anim. 1998 Jul;47(3):159-66. doi: 10.1538/expanim.47.159.
Age-related changes in major lymphocyte subsets were analyzed in 195 cynomolgus monkeys (Macaca fascicularis) aged from one month to 31 years. The percentages of CD20+ B cells in peripheral blood lymphocytes (PBL) decreased with age to five years of age, but after that, no significant change was observed. The percentages of CD16+ NK cells gradually increased during the first five years and reached the peak at from four to ten years of age, whereas the percentages of CD3+ T cells in PBL were relatively constant throughout the life. Among the T cells, the CD4+ CD8- T cells decreased, but CD4- CD8+ T cells increased within the first decade of life. We further analyzed the expressions of CD28 and CD29 molecules on T cells to determine the relation between age-related activation and phenotypic changes. Almost all CD4+ CD8- T cells (> 90%) were CD28+ at all ages analyzed, but a clear age-related decrease in CD28 expression was demonstrated in CD4- CD8+ T cells during the first ten years. In the case of CD29 expression, age-related increases in CD29hi cells were apparent in both CD4+ CD8- and CD4- CD8+ T cells during the first ten years. The percentages of CD29hi cells, however, were higher in CD4- CD8+ T cells than in CD4+ CD8- T cells in all ages analyzed. These results indicated that the age-related changes in percentages of major lymphocyte subsets as well as in phenotypes of T cells might be related to the maturation of the immune system including an increase in memory cells in cynomolgus monkeys.
对195只年龄从1个月到31岁的食蟹猴（猕猴）的主要淋巴细胞亚群的年龄相关变化进行了分析。外周血淋巴细胞（PBL）中CD20 + B细胞的百分比随年龄增长至5岁时下降，但此后未观察到显著变化。CD16 + NK细胞的百分比在最初5年逐渐增加，并在4至10岁时达到峰值，而PBL中CD3 + T细胞的百分比在整个生命过程中相对恒定。在T细胞中，CD4 + CD8 - T细胞减少，但CD4 - CD8 + T细胞在生命的第一个十年内增加。我们进一步分析了T细胞上CD28和CD29分子的表达，以确定年龄相关的激活与表型变化之间的关系。在所有分析的年龄中，几乎所有CD4 + CD8 - T细胞（> 90%）都是CD28 +，但在最初十年中，CD4 - CD8 + T细胞中CD28表达明显出现与年龄相关的下降。就CD29表达而言，在最初十年中，CD4 + CD8 - 和CD4 - CD8 + T细胞中CD29hi细胞与年龄相关的增加都很明显。然而，在所有分析的年龄中，CD4 - CD8 + T细胞中CD29hi细胞的百分比高于CD4 + CD8 - T细胞。这些结果表明，主要淋巴细胞亚群百分比以及T细胞表型的年龄相关变化可能与食蟹猴免疫系统的成熟有关，包括记忆细胞的增加。
