Krysiak B, Rydzyński K, Kieć-Swierczyńska M
Zakładu Toksykologii i Kancerogenezy, Instytutu Medycyny Pracy, Lodzi.
Med Pr. 1998;49(4):371-9.
The aim of the study was to determine the threshold irritating effect of benzalkonium chloride on the rabbit's skin and eye, and its sensitizing effect on guinea pigs. The study was performed according to the OECD guidelines modified by Krysiak. As a threshold concentration of benzalkonium evoking slight inflammatory reaction on the skin in a single, closed exposure and in multiple, open exposure, the following values were adopted for its aqueous solutions: > 0.5-1%, and 05% respectively. Following the eye administration of 0.01% and 0.05% of benzalkonium aqueous solution a weak irritating and rapidly reversible effect was revealed. The study performed on guinea pigs indicated a sensitizing effect of 0.5%, 0.1% and 0.05% benzalkonium aqueous solutions. The effect differed depending on the concentration used. The evaluation covered the proportion of sensitized animals, the nature and intensity of dermal reaction, as well as peripheral blood eosinophil and basophil tests. The results showed that 0.1% benzalkonium aqueous solution is an optimum concentration for monitoring the sensitizing effect in humans being in contact with this substance.
该研究的目的是确定苯扎氯铵对兔皮肤和眼睛的阈值刺激作用及其对豚鼠的致敏作用。该研究是根据Krysiak修改的经合组织指南进行的。作为苯扎氯铵在单次封闭暴露和多次开放暴露中引起皮肤轻微炎症反应的阈值浓度，其水溶液采用以下值：分别>0.5-1%和0.5%。眼部给予0.01%和0.05%的苯扎氯铵水溶液后，显示出轻微刺激且快速可逆的作用。对豚鼠进行的研究表明，0.5%、0.1%和0.05%的苯扎氯铵水溶液有致敏作用。该作用因所用浓度而异。评估涵盖致敏动物的比例、皮肤反应的性质和强度以及外周血嗜酸性粒细胞和嗜碱性粒细胞检测。结果表明，0.1%的苯扎氯铵水溶液是监测接触该物质的人类致敏作用的最佳浓度。
