Krysiak B, Barański B, Popińska E, Stetkiewicz J
Med Pr. 1980;31(5):379-92.
The experiment was carried out on 36 white Vienna rabbits and 27 grey rabbits of Chinchilla strain. The test substances were: sulphuric acid, acetic acid, sodium hydroxide and oil M-26. A single dermal 24 rh closed (sides) exposure, and single dermal open (sides, ears) exposure were used. The cutaneous reaction to the substances was evaluated according to Draize's et al. (1944) conventional point scale. In addition, cutaneous eruptions that have not been covered by the classification, have been included, with reactions in per cent. The intensification of the inflammatory reaction was found to depend on the way of application and duration of exposure to a given substance. The single open application resulted in cutaneous eruptions of a lesser intensity than the single closed application of the same compounds, the concentration being the same. The skin of white rabbits was found to be similarly sensitive to the irritating effect of the chemicals as the skin of grey rabbits. The histopathological examinations allowed determining the nature and localization of changes and revealed, in some cases, skin pathologies, with no clear symptoms of irritating effects of the substances revealed macroscopically.
该实验以36只白色维也纳兔和27只青紫蓝品系灰兔为实验对象。受试物质为：硫酸、乙酸、氢氧化钠和M - 26油。采用单次皮肤24小时封闭（双侧）暴露和单次皮肤开放（双侧、耳部）暴露。根据Draize等人（1944年）的传统评分标准评估皮肤对这些物质的反应。此外，还纳入了分类未涵盖的皮肤疹，并给出了反应百分比。研究发现，炎症反应的加剧取决于给药方式和对给定物质的暴露持续时间。在相同浓度下，单次开放给药导致的皮肤疹强度低于相同化合物的单次封闭给药。发现白兔皮肤对化学物质刺激作用的敏感性与灰兔皮肤相似。组织病理学检查能够确定变化的性质和部位，并且在某些情况下揭示了皮肤病理学特征，而肉眼未发现这些物质刺激作用的明显症状。
