天疱疮抗体对皮肤的体外作用。

In vitro effects of pemphigus antibodies on skin.

作者信息

Binder W L, Beutner E H, Chorzelski T P

出版信息

Br J Dermatol. 1978 Jul;99(1):39-42. doi: 10.1111/j.1365-2133.1978.tb01958.x.

DOI:10.1111/j.1365-2133.1978.tb01958.x

Abstract

Acantholysis occurring in rhesus monkey skin explants cultured on sera of 9 pemphigus patients was found to be largely dependent on the titre of intercellular antibody, and not on the participation of complement. Skin explants cultured on normal human sera and pemphigoid sera failed to give rise to intercullular staining or to develop lesions. Six of eight 'negative' pemphigus sera with intercellular antibody titres of less than 20 on skin (and titres ranging from 20 to 160 on monkey esophagus) reacted with the skin explants as revealed by direct immunofluorescence with an anti-IgG conjugate. The binding of antibodies from 3 of these 6 reactive sera resulted in some pathological changes in the explants. At least two of these 3 'negative' sera came from pemphigus patients with skin lesions.

摘要

在9例天疱疮患者血清中培养的恒河猴皮肤外植体中发生的棘层松解，很大程度上取决于细胞间抗体的滴度，而不是补体的参与。在正常人血清和类天疱疮血清中培养的皮肤外植体未能产生细胞间染色或形成病变。8份“阴性”天疱疮血清中有6份在皮肤上细胞间抗体滴度低于20（在猴食管上滴度范围为20至160），用抗IgG共轭物进行直接免疫荧光显示，这些血清与皮肤外植体发生反应。这6份反应性血清中有3份的抗体结合导致外植体出现一些病理变化。这3份“阴性”血清中至少有2份来自有皮肤病变的天疱疮患者。