Deng J S, Beutner E H, Shu S, Chorzelski T P
Arch Dermatol. 1977 Jul;113(7):923-6. doi: 10.1001/archderm.113.7.923.
The pathogenic effect of the intercellular antibodies of pemphigus was studied by using organ culture of monkey skin. Skin explants that were grown on sera with intercellular antibody titers of 320 or greater fixed these antibodies within one day as demonstrated by direct immunofluorescence for IgG. None of the control sera gave such staining patterns. Following the binding of intercellular antibodies, characteristic histologic changes appeared, notably separation of individual epidermal cells and acantholysis. These histologic changes became more marked in two to five days. During this period, the bound antibodies and the intercellular antigens decreased and disappeared. These temporal relationships of immunofluorescence and histologic findings suggest that pemphigus antibodies play a role in the induction of acantholysis.
采用猴皮肤器官培养法研究了天疱疮细胞间抗体的致病作用。用直接免疫荧光法检测IgG，结果显示，接种于细胞间抗体滴度为320或更高血清中的皮肤外植体在一天内即可固定这些抗体。对照血清均未出现此类染色模式。细胞间抗体结合后，出现特征性组织学改变，尤其是单个表皮细胞分离和棘层松解。这些组织学改变在2至5天内更加明显。在此期间，结合的抗体和细胞间抗原减少并消失。免疫荧光和组织学结果的这些时间关系表明，天疱疮抗体在棘层松解的诱导中起作用。
