Michel B, Ko C S
Br J Dermatol. 1977 Mar;96(3):295-302. doi: 10.1111/j.1365-2133.1977.tb06141.x.
An in vitro model for the study of pemphigus acantholysis has been developed. The histological changes of pemphigus vulgaris were reproduced in vitro in organ culture by growing normal human skin in the presence of pemphigus vulgaris or pemphigus foliaceus sera. At 24 h a suprabasilar split was noted and at 72 h extensive suprabasilar acantholysis developed. Direct immunofluorescent tests demonstrated that pemphigus antibody became bound to the epidermal intercellular space antigen(s) during the first 6-12 h. As acantholysis increased the presence of tissue-fixed antibody decreased. The fixation of the pemphigus antibody to the skin prior to the development of acantholysis provides strong evidence for the pathogenetic role of this antibody in the production of acantholysis. The data suggest that complement is not required in this model for the production of the acantholytic changes of pemphigus since heating the serum for 30 min at 56 degrees C did not destroy the acantholytic activity and no complement (C3) could be detected by DIF of organ culture explants.
已经建立了一种用于研究天疱疮棘层松解的体外模型。通过在寻常型天疱疮或落叶型天疱疮血清存在的情况下培养正常人皮肤，在器官培养中体外再现了寻常型天疱疮的组织学变化。在24小时时观察到基底上层分离，在72小时时出现广泛的基底上层棘层松解。直接免疫荧光试验表明，天疱疮抗体在最初6 - 12小时内与表皮细胞间空间抗原结合。随着棘层松解增加，组织固定抗体的存在减少。在棘层松解发生之前天疱疮抗体与皮肤的结合为该抗体在棘层松解产生中的致病作用提供了有力证据。数据表明，在该模型中产生天疱疮的棘层松解变化不需要补体，因为在56℃将血清加热30分钟不会破坏棘层松解活性，并且通过器官培养外植体的直接免疫荧光法未检测到补体（C3）。
