Wylie J L, Moses S, Babcock R, Jolly A, Giercke S, Hammond G
Cadham Provincial Laboratory, Department of Medical Microbiology, University of Manitoba, Canada.
J Clin Microbiol. 1998 Dec;36(12):3488-91. doi: 10.1128/JCM.36.12.3488-3491.1998.
We conducted a comparative evaluation of the Chlamydiazyme (Abbott Laboratories), PACE 2 (Gen-Probe), and AMP-CT (Gen-Probe) assays for the detection of Chlamydia trachomatis in endocervical samples. Specimens from 787 females were included in the study. The sensitivities of the PACE 2 and Chlamydiazyme assays in comparison to the results of the AMP-CT assay were 79.3 and 63.4%, respectively. The specificities of the Chlamydiazyme and PACE 2 assays were 100%. All of the positive specimens detected in this study were positive by the AMP-CT assay. On the basis of the final results of the comparison, the prevalence of C. trachomatis in the population was 10.4%. Retesting of specimens whose results were in the intermediate zone by the PACE 2 assay by a probe competition assay identified some additional true-positive specimens. Amplification assay testing of such specimens did not significantly increase the yield. The majority of specimens which tested positive by the AMP-CT assay only were not in the intermediate zone by the PACE 2 assay. We were unable to identify demographic or clinical factors which could predict those individuals who tested positive by amplified tests but not by nonamplified tests. The Gen-Probe PACE 2 assay proved to be superior to the Chlamydiazyme assay for the screening and diagnosis of C. trachomatis infections in female endocervical specimens.
我们对用于检测宫颈样本中沙眼衣原体的Chlamydiazyme（雅培实验室）、PACE 2（基因探针公司）和AMP-CT（基因探针公司）检测方法进行了比较评估。787名女性的样本被纳入该研究。与AMP-CT检测结果相比，PACE 2和Chlamydiazyme检测方法的灵敏度分别为79.3%和63.4%。Chlamydiazyme和PACE 2检测方法的特异性均为100%。本研究中检测到的所有阳性样本经AMP-CT检测均为阳性。根据比较的最终结果，该人群中沙眼衣原体的患病率为10.4%。通过探针竞争试验对PACE 2检测结果处于中间区域的样本进行重新检测，发现了一些额外的真阳性样本。对此类样本进行扩增检测并未显著提高检出率。大多数仅经AMP-CT检测呈阳性的样本，PACE 2检测结果不在中间区域。我们无法确定哪些人口统计学或临床因素能够预测那些经扩增检测呈阳性但未经非扩增检测呈阳性的个体。对于女性宫颈样本中沙眼衣原体感染的筛查和诊断，基因探针公司的PACE 2检测方法被证明优于Chlamydiazyme检测方法。
