Otton R, Graziola F, Hirata M H, Curi R, Williams J F
Department of Physiology and Biophysics, University of Sao Paulo, Brasil.
Biochem Mol Biol Int. 1998 Oct;46(3):529-36. doi: 10.1080/15216549800204052.
The effect of diets enriched with fat containing different fatty acids on the activity and expression of the glucose-6-phosphate dehydrogenase (EC 1.1.1.49) of mesenteric lymph nodes lymphocytes and intraperitoneal macrophages was examined. Measurements of the enzyme were also performed using spleen, thymus and liver for comparison. The following fat rich diets containing a variety of fatty acids were used: 1-standard chow (CC); 2-medium chain saturated fatty acids (MS)-coconut fat-oil; 3-long chain saturated fatty acids (LS)-cocoa butter; 4-monounsaturated fatty acids (MU)-canola oil (n-9); 5-polyunsaturated fatty acids (PU)-soybean oil (n-6). Of the fat-rich diets tested, MS had the least effect. The G6PDH activity of lymphocytes was reduced by all the fat-rich diets; 16% for MS, 38% for LS, and 54% for MU. Similarly, the enzyme activity was reduced in macrophages; 35%, 86%, and 73%, for LS, MU, and PU, respectively. In contrast, the fat-rich diets elevated G6PDH activity in the lymphoid organs; by 42% in the spleen due to LS and by 131%, 35%, and 56% in the thymus due to LS, MU, and PU, respectively. Fat-rich diets decreased the activity of G6PDH in liver; 42%, 68%, and 39% for MS, MU, and PU, respectively. Some of the changes in G6PDH activity induced by the fat-rich diets occur through the mechanisms of mRNA abundance.
研究了富含不同脂肪酸的饮食对肠系膜淋巴结淋巴细胞和腹腔巨噬细胞中葡萄糖-6-磷酸脱氢酶（EC 1.1.1.49）活性和表达的影响。还使用脾脏、胸腺和肝脏进行酶的测量以作比较。使用了以下含有各种脂肪酸的富含脂肪的饮食：1-标准饲料（CC）；2-中链饱和脂肪酸（MS）-椰子油；3-长链饱和脂肪酸（LS）-可可脂；4-单不饱和脂肪酸（MU）-菜籽油（n-9）；5-多不饱和脂肪酸（PU）-大豆油（n-6）。在所测试的富含脂肪的饮食中，MS的影响最小。所有富含脂肪的饮食均降低了淋巴细胞的G6PDH活性；MS降低了16%，LS降低了38%，MU降低了54%。同样，巨噬细胞中的酶活性也降低了；LS、MU和PU分别降低了35%、86%和73%。相反，富含脂肪的饮食提高了淋巴器官中G6PDH的活性；LS使脾脏中的活性提高了42%，LS、MU和PU分别使胸腺中的活性提高了131%、35%和56%。富含脂肪的饮食降低了肝脏中G6PDH的活性；MS、MU和PU分别降低了42%、68%和39%。富含脂肪的饮食诱导的G6PDH活性的一些变化是通过mRNA丰度机制发生的。
