Shapiro S D
Department of Pediatrics, Washington University School of Medicine, Barnes-Jewish Hospital, St Louis, MO 63110, USA.
Curr Opin Cell Biol. 1998 Oct;10(5):602-8. doi: 10.1016/s0955-0674(98)80035-5.
Targeted mutagenesis has allowed investigators to perform controlled experiments in mammals and determine the contribution of individual proteins to physiologic and pathologic processes. Recent lessons learned from matrix metalloproteinase gene targeted mice and other in vivo observations have given new life to old concepts regarding the role of proteolytic fragments of extracellular matrix proteins in regulating a variety of critical processes in cell biology.
靶向诱变使研究人员能够在哺乳动物中进行对照实验，并确定单个蛋白质在生理和病理过程中的作用。最近从基质金属蛋白酶基因靶向小鼠和其他体内观察中吸取的经验教训，为细胞生物学中细胞外基质蛋白的蛋白水解片段在调节各种关键过程中的作用这一古老概念赋予了新的活力。
